Animal-free Recombinant Human IL-12 is expressed in human 293 cells as a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). Production in human 293 cells uses a serum-free, chemically defined media and offers authentic glycosylation. IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.