Memory T cells are antigen-exposed T lymphocytes which would mediate fast and robust response upon repeat encounter with antigen. Human CD4 Memory T Cell Isolation kit is designed to isolate untouched CD4+ memory T lymphocytes which contains a biotin labeled antibody cocktail and streptavidin conjugated magnetic beads. Through incubation of samples with biotin antibody cocktail followed by incubation of streptavidin beads, the magnetically labeled non CD4+ memory cells will be retained in the tube by magnetic separator. The untouched CD4+ T memory cells are collected in supernatant. Any downstream application can be performed on CD4+ memory cells after separation, such as Flow Cytometry and in vitro culture.