CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. 45%-70% human peripheral blood mononuclear cells (PBMC) express CD3. Human CD3 Magnetic Beads Kit is used for isolation or depletion of human CD3 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD3 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD3+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD3- cells remain in supernatant and can also be used for further applications.