CD14 is a surface antigen expressed on monocyte/macrophage. It cooperates with other proteins to mediate innate immune response to bacterial LPS. 5%-10% human peripheral blood mononuclear cells (PBMC) express CD14. Human CD14 Magnetic Beads Kit is used for isolation or depletion of human CD14 monocytes/macrophages from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD14 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD14+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD14- cells remain in supernatant and can also be used for further applications.