KE00127 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The BMP7 ELISA kit is to be used to detect and quantify protein levels of endogenous BMP7. The assay recognizes human BMP7. An antibody specific for BMP7 has been pre-coated onto the microwells. The BMP7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human BMP7 is added to detect the captured human BMP7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.