Glycerol is a simple polyol compound which is a viscous liquid that is widely used in pharmaceutical formulations. It has three hydroxyl groups that are responsible for its solubility in water and its hygroscopic nature. It is a trihydroxy sugar alcohol that is an intermediate in carbohydrate and lipid metabolism. It is used as a solvent, emollient, pharmaceutical agent, sweetening agent and is of low toxicity.
Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.
Grade: Molecular Biology Reagent
Purity: ≥99%
Key Applications: Protein purification, cell culture, enzyme assays
Product Type: Solvents
Biochemical Category: Polar
Density: 1.2613 g/cm3 at 20°C (Lit.)
Auto Ignition: 412°C at 1 atm.(Lit.)
Boiling Point: 166.1°C at 13.3 hPa(Lit.)
Melting Point: 18.2°C (Lit.)
Vapor Density: 3.17 (air = 1) (Lit.)
Vapor Pressure: .0033 hPa at 50°C (Lit.)
Refractive Index: n20/D 1.474(lit.)
Presentation: Liquid
pH: 5.0 - 7.5 (1% aq solution)
Heavy Metals: Lead <5 ppm
Solubility: 1 mL/mL of water, clear solution.
Storage & Handling: Store at Room Temperature (15-30°C)