First strand cDNA synthesis kit is in a 5x master mix form which contains Reverse Transcriptase (RT), recombinant RNase inhibitor, dNTPs and an optimized buffer with protein stabilizers. G-biosciences RT is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. This RT is an RNA-directed DNA polymerase which lacks 3´→ 5´ exonuclease activity and can produce full-length cDNA from long RNA templates.
Product Includes:
Applications:
- First strand cDNA synthesis
- Gene expression analysis
Benefits:
- Active at temperatures up to 55°C.
- Highly efficient at producing full-length cDNA from low RNA concentration.
Storage Temperature: – 20º C