Erythromycin is a macrolide antibiotic that bind to the 50S ribosomal subunit and inhibit protein synthesis. This is an antimicrobial substance produced by the growth of certain strains of Streptomyces erythreus. It is a mixture consisting largely of erythromycin A with lesser amounts of erythromycins B and C.
Used in cell culture applications at approximately 100 mg/liter. Depending upon the strain of bacteria, erythromycin has been used between 50 and 200 mg/L to control bacterial growth. Erythromycin resistance can be induced in bacteria.
Mode of Action: Inhibits protein synthesis (elongation) at the level of transpeptidation (aminoacyl translocation A-site to P-site). Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
Purity: ~98%
Potency: ≥850 µg/mg
Key Applications: Antibiotic
Application Areas: Cell Biology & Analysis
Product Type: Biochemicals
Biochemical Category: Antibiotics
Chemical Class: Antibiotics
Melting Point: 135 to 140°C (Lit.)
Extinction Coefficient (E1%): EM = 50 (280 nm, pH 6.3)
Optical Rotation: -71° to -78° (c=2, ethanol)
Presentation: White Powder
Format: Powder
pH: 8.0 - 10.5 (70% aq soln)
pKa: 8.8
Detection Method: FTIR
Moisture content: <10%
Solubility: Slightly soluble in water (~2 mg/mL); Moderately soluble in ether, ethylene dichloride, amyl acetate; freely soluble in alcohols (ethanol - 50 mg/mL), acetone, chloroform, acetonitrile, ethyl acetate. Soluble in 2 M Hydrochloric acid (50 mg/mL).
Storage & Handling: Store at Room Temperature (15-30°C).