For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
- Sensitive and accurate. Use as little as 20 μL samples. Linear detection range in 96-well plate: 0.1 to 3 mM (1.8 mg/dL to 54 mg/dL) glucose for colorimetric assays.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at 20 minutes. Room temperature assay. No 37°C heater is needed.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
- Method: OD340nm
- Samples: Serum, plasma, urine, saliva, milk, culture medium, food, beverages etc
Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit II uses an enzyme to reduce NAD. The produced NADH, measured at 340 nm, is proportional to the glucose concentration in the sample