Cholesterol Esterase is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
- Stored intracellularly as cholesterol esters
- Reversible enzyme
- Hydrolyzes and synthesizes fatty acid esters of cholesterol and other sterols
Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The enzyme may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.
One unit is the amount of enzyme that will hydrolyze 1.0 micromole of cholesterol ester per minute at 37°C, pH 7.0.
Specific Activity: ≥100 u/mg solid
Key Applications: Synthesis of fatty acid esters of cholesterol
Application Areas: Proteomics, Diagnostics, Enzymes, Inhibitors
Product Type: Proteins, Enzymes & Peptides
Protein or Enzyme Type: Proteins, Enzymes & Peptides
Presentation: Yellowish Tan Lyophilized Powder
Format: Lyophilized Powder
Isoelectric point (pI): 5.9 ± 0.1(Lit.)
pH: Optimum pH: 7.0 - 9.0 ; pH Stability: pH 5.0 - 9.0 (25°C, 24hr)
NOTES: Stabilizers: Mg- - Na-cholate, bovine serum albumin; Inhibitors: Hg- - Ag ionic detergents; Michaelis constants:5.4 x 10-5M(Linoleate), 6.6 x 10-5M(Oleate); 3.7 x 10-5M(Lnolante), 1.5 x 10-5M(Palmitate); 1.2 x 10-5M(Myristate), 2.3 x 10-5M(Stearate); Thermal Stability: below 55°C (pH 7.5, 10 min); Optimum temperature: 40°C
Solubility: Soluble in distilled water or dilute buffer.
Storage & Handling:-20°C