Carcinomas arising from epithelial cells lining the mammary glands comprise the overwhelming majority of breast cancer cases (1). Transformation of mammary epithelial cells from their normal non-proliferative condition to an immortal state enabling hyperproliferation and malignancy occurs via bypass of senescence checkpoints. Cellular models that allow study of the mechanisms giving rise to malignancy are of paramount importance to the identification of therapeutic targets for breast cancer treatment.The C57MG immortalized mammary epithelial cell line is an established model for mammary epithelial malignancy. C57MG cells are syngeneic with the C57BL/6 mouse model and have demonstrated potential of transformation with many proto-oncogenic factors. The C57MG cell line is widely used in studies of the role of Wnt signaling in malignant transformation (4). C57MG cells have been shown to upregulate expression of proto-oncogenic beta-catenin in response to Wnt proteins (5). The features of the C57MG cell line make it a highly relevant model system for studies of breast cancer malignancy. Source:The C57MG cell line was established from mammary epithelial tissue of a C57BL/6 mouse (2).Research Category:Cancer