The gene ADP-ribosylation factor GTPase-activating protein-2 (ARFGAP2) has been mapped to human chromosome 11p11.2. ARFGAPs form a family of proteins sharing a conserved catalytic domain which include a zinc finger motif, and they differ in the non-catalytic domains. Human genome contains sixteen genes coding for ARFGAPs. Fluorescent protein tagging showed ARFGAP2 localization on the Golgi complex and punctuate structures in the cytoplasm identified as endoplasmic reticulum-Golgi intermediate compartments/vesicular-tubular compartments.
Synonyms: Anti-ADP-ribosylation factor GTPase-activating protein 2; Anti-ARF GAP 2; Anti-GTPase-activating protein ZNF289; Anti-Zinc finger protein 289
Storage: -20C
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Biochem Physiol Actions: ADP-ribosylation factor GTPase-activating protein-2 (ARFGAP2) is shown to associate with COP-I-coated vesicles produced in-vitro. Additionally, it is shown to be involved in the COP-I-dependent Golgi-to-endoplasmic reticulum trafficking of a model cargo, cholera toxin. ARFGAP1, 2 and 3 together are essential for COP-I-mediated trafficking in mammalian cells. Triple knockout of ARFGAP1/2/3 in HeLa cells result in cell death. Single knockdown of ARFGAP2 in HeLa cells using siRNA approach results in Golgi unstacking and cisternal shortening. Brefeldin A treatment results in re-distribution of the ARFGAP2 from the Golgi complex to the cytosol.
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