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Jackson Immunoresearch

Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 fragment specific

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Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 fragment specific

F(ab')2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region. F(ab')2 fragments have two antigen-binding Fab portions linked together by disulfide bonds and therefore they are divalent. The average molecular weight is about 110 kDa. They are used for specific applications, such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the F(ab')2/Fab portion of human IgG. It also reacts with the light chains of other human immunoglobulins. No antibody was detected against the Fc portion of human IgG or against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.
 

Target: Human

Host: Goat

Antibody Format: F(ab')2 Fragment

Specificity:  IgG, F(ab')2 fragment specific

Minimal Cross Reactivity: Bovine, Horse, Mouse Serum Proteins

Conjugate: Alexa Fluor® 488

Clonality: Polyclonal

Physical StateFreeze-dried powder
StorageStore freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing. 
Expiration dateone year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.
PurityThe antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative0.05% Sodium Azide 
Suggested Working Concentration or Dilution Range
Histo-/Cyto-chemistry- 1:100-1:800
Flow Cytometry- 1:100-1:800
Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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Product Detail
Thomas No.
C840L81
Mfr. No.
109-546-097
Description
Alexa Fluor® 488 AffiniPure F(ab')2 Fragment Goat Anti-Human IgG, F(ab')2 fragment specific, 0.75 mg
list price/quantitytotal
$0.00
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