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HiMedia

Agar Powder, Purified

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HiMedia’s Agar Powder, Purified meets the requirements for all purpose gelling agent used in wide range of microbial culture media. It is prepared under the strictest hygienic conditions. Ash content of it can be relied upon to provide a source of trace minerals required for optimum growth of most microorganisms, without yielding precipitate following sterilization in presence of other media constituents.

Appearance
Cream colored, Homogenous Free flowing powder.

Solubility
Freely soluble in hot water at temperatures above 85°C. Insoluble cold water.

Clarity
A firm solid, clear to slightly opalescent gel is formed at a concentration of 1.5% at 36-38°C.

Dye Diffusion
Agar dye diffusion :- 18-20mm

Reaction
Reaction of 1.5% w/v aqueous solution at 25 °C.

Storage and Shelf Life
Store below 30°C. Use before expiry date on the label.

pH6.50- 7.50
Identification testAs per method specified in USP 31, NF26. With Iodine some fragments of agar appear bluish black with some areas reddish to violet. Agar forms a clear liquid, which congeals at 32 to 39°C to form a firm resillent gel, which does not melt below 85°C.
Total aerobic microbial count (cfu/gm)By plate method when incubated at 30-35°C for not less than 3 days.
Total aerobic microbial count : <= 2000 CFU/gram
Total Yeast and mold count (cfu/gm)By plate method when incubated at 20-25°C for not less than 5 days.
Yeast & mold Count : <= 100 CFU/gram
Test for Pathogens1. E.coli-Negative in 10 gms of sample 2. Salmonella species-Negative in 10 gms of sample 3. Pseudomonas aeruginosa-Negative in 10 gms of sample 4. Staphylococcus aureus- Negative in 10 gms of sample 5. C.albicans- Negative in 10 gms of
sample 6. Clostridia- Negative in 10 gms of sample
Cultural RepsonseRM301: Cultural response observed after an incubation at 35-37°C for 18-24 hours by preparing Nutrient Agar (M001) using Agar Powder,Purified as an ingredient.

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Product Detail
Thomas No.
C985J85
Mfr. No.
RM201-500G
Description
Agar Powder, Purified, 500g
list price/quantitytotal
$0.00
$0.00 (0 Items)
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