RNA to cDNA to qPCR, in one tube
- High purity enzyme formulation for enhanced stability and performance
- Accuris Hot-Start Taq allows for preparation at room-temperature
- Blue dye facilitates pipetting and visualization in plates
- Available for green fluorescence or probe detection
- Multiplex formulation available for multiple target amplification
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.