5-Chloro-2'-deoxyuridine (CldU) is a thymidine analog that is readily incorporated, following phosphorylation, into newly synthesized DNA in place of thymidine.
5-Chloro-2′-deoxyuridine (CldU) is used as a thymidine analogue to study the miscoding potential of hypochlorous acid damage to DNA and DNA precursors. When used with antibody based immunofluorescent imaging, 5-Chloro-2′-deoxyuridine incorporation may be used in protocols to identify sites of DNA replication. CldU may be used as a labeling substrate in conjunction with other halogenated uridine labeling substrates such as bromodeoxyuridine (BrdU) and/or iododeoxyuridine (IdU).
Key Applications: Biochemical, Analogue
Product Type: Biochemicals
Biochemical Category: Nucleotides & Nucleic Acids
Melting Point: 166 - 176°C
UV/Visible Absorbance: λ max (water): 276 ± 3 nm
Presentation: White Powder
Solubility: Soluble in 1M NH4OH (20 mg/1 mL-colorless solution).
Storage & Handling: -20°C