AEBSF hydrochloride is a covalent binding serine protease inhibitor, belongs to the family of irreversible sulfonyl fluoride inhibitors that block trypsin and chymotrypsin-type enzymes. Similar in structure to the commonly used inhibitor PMSF, AEBSF offers better solubility in water, lower toxicity, greater stability and higher inhibitory activity.
The lower molecular weight and toxicity of AEBSF relative to PMSF and related organophosphate inhibitors, make this inhibitor well suited for blocking trypsin-like activities in tissue culture media as well as in large scale purification of recombinant proteins , susceptible to proteolysis.