The lung epithelium undergoes a complex series of endoderm–mesoderm-mediated signaling events to generate a complex arborized network of conducting airway (bronchi, bronchioles) and gas-exchanging units (alveoli). Lung epithelium differentiation stages include: anterior/posterior and dorsal/ventral patterning, lung specification, lung budding, branching morphogenesis, and maturation. The 3dGRO(TM) Lung Organoid Branching Medium is a chemically defined/serum-free ready-to-use media used to differentiate Sox2+/Pax6+ anterior foregut endoderm (AFE) cells into lung bud organoids in suspension cultures. Additionally, the 3dGRO(TM) Lung Organoid Branching Medium can be used to culture AFE cells on fibronectin coated cultureware. The 3dGRO(TM) Lung Organoid Branching Medium is a component of the 3dGRO(TM) Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO(TM) Human Lung Organoid Culture System includes (all sold separately): • Definitive Endoderm Induction Medium (SCM302)• Anterior Foregut Endoderm Induction Medium I (SCM305)• Anterior Foregut Endoderm Induction Medium II (SCM306)• 3dGRO(TM) Lung Organoid Branching Medium (SCM307)• 3dGRO(TM) Lung Organoid Maturation Medium (SCM308)Human iPSC Derived AFE Progenitors (SCC301)
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