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This 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12 media is ideal for culture of a variety of cell types under low serum conditions. Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the...

Phosphate buffered saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate, and in some formulations potassium chloride and potassium phosphate. PBS helps to maintain a constant pH and is isotonic and non-toxic to cells. This formulation does not contain calcium and magnesium....

Miller's LB broth is based on the original Lysogeny Broth (LB) media formulation developed by Luria and Bertani, which was designed for the rapid propagation of recombinant E. coli strains.  Miller's LB contains a 20-fold concentration sodium chloride compared to the original...

Wide range of applications in processes requiring high levels of sterility Meets WFI Standards Produced under the highest industry standards to ensure superior results This Water for Injection (WFI)-quality water meets United States Pharmacopeia (USP) testing requirements.  Applications for...

Lower documented Endotoxin content then offerings from other major suppliers 100 mM Solution with 8.5 g/L NaCI Produced under the highest industry standards to ensure superior results Sodium pyruvate is often used as a component in culture broth and media as an energy source for propagating...

L-glutamin L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell...

The Corning® Mediatech™ cell culture media is formulated with high industrial standards for excellent results. It maintains cell tonicity and viability. Application: For Research Use Only

TBE (Tris-Borate-EDTA) has applications in DNA and RNA agarose gel electrophoresis and polyacrylamide gel electrophoresis.  TBE has a higher buffering capacity than Tris-Acetate-EDTA (TAE) buffer, but the latter is more suitable for steps involving the action of enzymes on nucleic acids because...

Dulbecco's Phosphate Buffered Saline (DPBS) is a buffer solution which can be used to maintain cell culture media in the physiological pH range of 7.0 to 7.6. It can serve as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time....

Dimethyl Sulfoxide (DMSO) is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing. Complete Certificate of Analysis available each production lot, along with full formulation information Produced under the highest industry standards to...

Ham's F-12 media was originally formulated for the serum-free culture of CHO cells, and is based on Ham's F-10 medium with increased concentrations of choline, inositol, putrescine, and several amino acids.  This medium is also suitable for carcinoma cells, rat skeletal myoblasts,...

Named for the site at which it was developed, the Roswell Park Memorial Institute, RPMI 1640 is a modification of McCoy's 5A containing an increased level of inositol. Examples of cell types for which this medium is appropriate include mammalian myeloma and hybridoma cells such as mouse...

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer.  Linear, double-stranded DNA separates faster in a TAE buffer-based system compared to Tris-Borate-EDTAA (TBE) buffer, but the latter has a higher buffering capacity...

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.  TBS can shift pH relatively dramatically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in...

Technical Advantage:  Effective against Gram-positive and Gram-negative bacteria This product is a mix of the antibiotics Penicillin (10,000 IU) and Streptomycin (10,000 µg/ml) in a 100-fold working concentration. Penicillin (Penicillin G) works by inhibiting peptidoglycan synthesis,...

Dulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME. The original formulation was used to culture embryonic mouse cells and has since been modified to support a variety of...

Minimum Essential Medium (MEM) is a common cell culture medium developed from early work using Basal Medium Eagle (BME) with normal mammalian fibroblasts and certain subtypes of HeLa cells. This research indicated that additions made to BME aided cell propagation. MEM is modified with higher...

Phosphate Buffered Saline is used to maintain a physiological pH and osmolarity, two key factors in tissue culture and molecular biology research. This concentrated form should be diluted to a working concentration using reagent grade water. Technical Advantage: Product manufactured under...

Technical Advantage:  Lower documented Endotoxin content then offerings from other major suppliers. Hanks' Balanced Salt Solution (HBSS) is a buffer used to maintain a physiological pH for cells maintained in non-CO2 atmospheric conditions.  This formulation is produced without sodium...

Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging.  Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action.  EDTA sequesters these ions and thus enhances the efficacy of...

Technical Advantage:  Suitable for a wide variety of routine tasks requiring a high degree of sterility Molecular biology grade water is ideal for the preparation of reagents, rinsing glassware and plasticware, and other molecular biology applications where Rnase, Dnase, and Protease-free water...