LGC, Biosearch Technologies

Chemically competent E. coli engineered to regulate the copy number of CopyControl Fosmid clones.

CopyCutter™ EPI400™ E. coli cells will significantly lower the copy number of a wide variety of common vectors to enable cloning of unstable DNA sequences and those expressing toxic proteins

This reagent is designed for use with the MasterPure™ kit protocols only.

phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only...

T7 RNA Polymerase catalyzes the 5'′→3' RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.

EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT), an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable...

Electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.

The Poly(A) Polymerase Tailing Kit provides the enzyme and other reagents for quickly and easily adding a poly(A) tail to the 3´ end of any RNA. Poly(A) Polymerase is encoded by an E. coli gene that has been cloned into a plasmid and overexpressed in an E. coli strain.

Lucigen’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be...

The MasterPure™ Complete DNA and RNA Purification Kit enables rapid, high yield purification of high-molecular-weight genomic DNA, total cellular RNA and Total Nucleic Acid (TNA) from many different sample types.

Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of...

Perfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library...

Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3´′→5´ exoribonuclease that digests essentially all linear RNAs but does not digest lariat or circular RNA structures.

RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.

Hybridase™ Thermostable RNase H specifically degrades the RNA in a DNA:RNA hybrid, without affecting DNA or unhybridized RNA.

RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min...

RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.

EconoTaq’s low price is coupled with high quality and performance. QC specifications for EconoTaq are rigorous: Greater than 99% pure by SDS gel electrophoresis. No detectable DNA contamination as determined by PCR using generic primers. No detectable endonuclease (nicking) activity....

Chemically Competent E. coli cells are ideal for most cloning applications. The cells provide very high transformation efficiency.

This reagent is designed for use with the MasterPure™ kit protocols only.

EpiScript™ Reverse Transcriptase an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with reduced RNase H activity

Rec J Exonuclease, derived from E. coli, catalyzes degradation of ssDNA in a 5´′→3´ direction. Its activity is dependent on Mg2+. Rec J Exonuclease can be heat-inactivated by incubation at 65°C for 20 minutes.

TransforMax™ EC100™ Electrocompetent E. coli competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency.