IBI Scientific

Tetracycline Hydrochloride inhibits protein synthesis (elongation) by preventing the binding of aminoacyl-tRNA to ribosome. Bacteriostatic. Antibiotics dissolved in ethanol need not be sterilized. Magnesium ions are antagonists of tetracycline. Use media without magnesium salts (e.g. LB medium) for...

Glycerol is used to prevent freezing during enzyme and bacterial preparations.

IBI’s Proteinase K Solution is a stable 20mg/ml highly active proteolytic enzyme that is purified from the mold Tritirachium album. The enzyme has two binding sites for Ca++, which lie some distance from the active site and is not directly involved in the catalytic mechanism. The high activity...

Isopropanol is widely used for precipitating nucleic acids. The nucleic acid precipitate, which is formed in the presence of moderate concentrations of monovalent cations, is recovered by centrifugation and redissolved in an appropriate buffer at the desired concentrations.

Streptomycin Sulfate is used to bind to S12 protein and 30S ribosomal subunit, inhibits translocation, and elicits miscoding batericidal. NOTE: Stock solution of antibiotics dissolved in water should be sterilized by filtration through a 0.22 micron filter. Store at +4°C. Warm to room...

EDTA is commonly used in biological and electrophoresis buffer systems. Store at room temperature. Protect from moisture. Irritant. Causes irritation to eyes, skin and mucous membranes. Avoid breathing dust. Do not take internally. Wash thoroughly after handling. See Material Safety Data Sheet for...

Sucrose is used in rate-zonal centrifugation to separate molecules, based on size and conformation. Formula: N/A Formula Weight: N/A CAS Number: 57-50-1 Synonym(s): Beet sugar, Cane sugar

DTT is used in enzyme preparations to prevent formation of disulfides from sulfhydryl groups, thereby stabilizing the enzyme. Note: Do NOT autoclave DTT or solutions containing DTT

X-GAL is histochemical substrate for β-galactosidase, allowing the detection of lac gene expression. To make a stock solution of X-GAL, dissolve the X-GAL in dimethylformamide to make a 20mg/ml solution. Note: Use either a glass or polypropylene tube. Add 4ml of this solution per liter of...

IBI replacement Total RNA columns can be used with most kits on the market today. The glass fiber matrix columns are standard in most kits utilizing a lysis and binding method for purifying RNA. The user must observe column specifications for sample size, molecule size, and elution volume.

IBI replacement DNA columns and collection tubes are for use with IBI Genomic DNA Extraction kits or comparable competitive Genomic DNA Extraction kits that utilize the Lysis, bind, wash and elution method. These replacement columns and collection tubes are ideal for using up extra reagents from...

Ultra-pure and large-volume plasmid preparation IBI’s MIDI and MAXI Plasmid Kits use pre-packed, ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate...

The PR-10 pipette filler stand is a colorful utility stand for your lab’s pipette fillers. It is constructed of sturdy and durable 1/4” cast acrylic for years of continued use. The 1” diameter hole accepts most mechanical pipette fillers with ease. The skid-proof rubber feet also...

IPTG is an inducer of β-galactosidase activity in E. coli. It is commonly used in conjunction with X-GAL to detect lac gene expression in cloning applications, thus allowing detection of recombinant molecules.

Gel Size: 10cm(w) x 15cm(l) Buffer volume: 500ml The MP1015 is the ideal multi-purpose gel box for both extended high resolution, linear runs, and economical quick screens. The MP1015 contains buffer recirculation ports for long runs requiring recirculation. The MP1015 is constructed of 3/8"...

IBI’s PR-40 and PR-42 have four fluorescent orange angled shelves that ensure your pipettes remain safely in place, while adding a touch of color to your lab. The PR-40 and PR-42’s notched shelves also offer easy access to many different pipette lengths. Depending on the size of the pipette, the...

Ready-to-use DNA Ladder (pre-mixed with loading dye) Suitable for sizing double-stranded DNA from 100 bp to 3 Kb with enhanced intensity at 500 bp C852A58: Concentration: 2.5 µg / 25 µl; (Recommended loading: 0.5 µg or 5 µl) C852A59: Concentration: 50 µg / 500 µl;...

Methylene Blue is a recommended stain for agarose gels when recovery of DNA is required. The benefit of using methylene blue (rather than ethidium bromide) is the ability to use a white light box for visualization and photography in place of a UV light box. Using a white light box eliminates...

Chloramphenicol is used in the amplification of plasmids and colony hybridizations. Antibiotics dissolved in ethanol need not be sterilized. Store at +4°C. Warm to room temperature before opening. Keep tightly sealed. Store Chloramphenicol solution in a light-tight container at –20°C...

For use in buffers and cell culture media Sodium chloride is an ionic compound with the molecular formula of NaCl. Sodium chloride is the salt found most in the extracellular fluid of many multicellular organisms. It is also commonly used as the main component in saline solution. This material is of...

Sterile Endotoxin Free pH = 8.3 IBI TE buffer is packaged in medical grade non-leaching polycarbonate bottles to ensure purity levels and sterility are maintained. This 1X TE Buffer is certified nuclease free and endotoxin free by way of LAL testing to < 0.05 EU/ml. This 1X TE buffer has a pH of...

MICROINJECTION FISH MOLD-60 WELLS

Total RNA Minipreps from cultured cells and blood samples The Total RNA MINI and MAXI Kits (blood and cultured cell) are specially designed for purification of total RNA from fresh whole human blood and cultured cells. This method uses detergents and a chaotropic salt to lyse cells and inactivate...