Biotium

Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM...

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent...

FITC (fluorescein-5-isothiocyanate) is an amine-reactive fluorescein derivative for labeling proteins or other molecules containing primary amines.    &lamba;_Ex/&lamba;_Em= 492/520 nm   ε = 75,000   Orange solid soluble in DMF or DMSO   Store at...

This product is prepared by labeling an affinity-purified chicken anti-rabbit IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent...

ViaFluor® SE Cell Proliferation Kits use amine-reactive dyes to covalently label cells throughout the cell cytoplasm and intracellular compartments. Cell proliferation dyes are commonly used to monitor cell division by flow cytometry. The dyes also can be used to stably label cells to image cell...

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with CF®555 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange-red...

CD63 (clone H5C6) is a mouse monoclonal antibody that recognizes the human tetraspanin protein CD63. This antibody has been validated in flow cytometry, in immunofluorescence microscopy, and in western blot. It is supplied at 0.5 mg/mL, BSA-free.

Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies. Fish gelatin can be used in...

Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~ 0.1 uM to >100 uM. Renilla luciferase has been widely used as...

Goat anti-guinea pig IgG (H+L) labeled with green fluorescent CF®488A dye (excitation/emission 490/515 nm). The antibody has been cross-adsorbed against bovine, chicken, goat, Syrian hamster, horse, human, mouse, rabbit, sheep and rat serum. 1 mg IgG lyophilized with 7.5 mg BSA and 10 mg trehalose...

Flubida-2 has been used to detect pH at a specific site in a cell such as cell organelles by directing the probe to where avidin fusion proteins are located. The probe is a conjugate of biotin and fluorescein diacetate, which is nonfluorescent until the probe has entered the cells and hydrolyzed by...

This product is prepared by labeling highly cross-adsorbed goat anti-rabbit IgG (H+L) with CF®770 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared...

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has...

For researchers wishing to investigate the suitability of our various chromogenic beta-glucuronidase substrates, we offer this sampler kit, which contains 5 mg each of the following three different substrates: X-GlcU, CHA, Red-beta-D-GlcU, CHA, and Rose-beta-D-GlcU, CHA.

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.    Off-white solid soluble in DMSO   Store desiccated at -20°C   C₂₅H₁₆O₉...