Biotium

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an...

MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) is a tetrazolium salt that is commonly used to detect reductive metabolism in cells for viability, proliferation and cytotoxicity assays. On reduction tetrazolium salts turn into deeply colored products that can be easily quantified...

Goat anti-mouse IgM (μ chain) labeled with CF®680R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. NIR fluorescent CF®680R dye has excitation/emission at 680/701 nm....

LysoView™ 550 is an orange fluorescent lysosome stain for live cells, or detection in the Cy®3 channel. The dye has excitation/emission at 542/567 nm under acidic conditions. It is a replacement for LysoView™ 540 dye, with improved photostability. Supplied at 1000X in DMSO, 50 uL.

This product is prepared by labeling high quality goat anti-swine IgG (H+L) with CF®568 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF®568 dye has...

The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414, but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while...

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. The kits contain PMA dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public...

This product is prepared by labeling high quality rabbit anti-mouse IgG (H+L) with CF®640R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®640R dye...

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF®660C dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

Cyanine 647-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. Far-red fluorescent Cyanine 647 is structurally identical to Cy®5 and has excitation/emission at 650/665 nm). Cy dye is a registered trademark of GE Healthcare.

Z-FR-AMC is a substrate for serine proteases, including cathepsins, kallikrein and plasmin. λ_Ex/λ_Em of substrate = 330/390 nm (weak fluorescence) λ_Ex/λ_Em of end product (AMC) = 342/441 nm (strong fluorescence) Off-white solid...

5-ROX-PEO₈ SE (full name: 5-Carboxy-X-rhodamine-PEO₈-propionate, succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates the red fluorescent ROX dye and the amine-reactive SE group. &lamba;_Ex/&lamba; _Em(MeOH) = 568/595...

The amine reactive succinimidyl ester of EB (ethidium bromide) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups. The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in...

Green-Beta;-D-Gal (N-Methylindolyl-beta-D-galactopyranoside) is a chromogenic substrate for β-galactosidase. Similar to X-Gal, but generates green colored precipitate on enzyme hydrolysis. White solid soluble in water and DMSO Store at 4°C and protect from light...

BCIP, toluidine (5-Bromo-4-chloro-3-indoxyl phosphate, p- toluidine salt) is a widely used chromogenic phosphatase substrate, which forms a dark blue (λ_max 615 nm) precipitate on enzymatic hydrolysis. It is often used with the oxidant NBT (nitro blue tetrazolium chloride), which...

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-mouse IgG (H+L) with R-phycoerythrin (R-PE or PE).

Aminooxy-biotin can be used to covalently attach biotin to a molecule with an aldehyde or ketone group. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage in an aqueous solution at neutral pH. The reaction is fast and can be further accelerated by adding...

BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an...

This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A...

Fura-2, AM ester is a ratiometric fluorescent dye that binds to free intracellular calcium, and is the preferred dye for ratiometric imaging microscopy. Fura-2, AM ester is membrane-permeant and thus can be loaded into cells via incubation. Because of the relatively low water solubility of the AM...

Ultra-pure LE Agarose has low electroendoosmosis (EEO) for high electrophoretic mobility. This agarose has excellent performance for analytical or preparative nucleic acid electrophoresis and blotting, and is validated for use with Biotium's GelRed®, GelGreen®, and DNAzure® gel stains.

This product is prepared by labeling high quality F(ab')2 fragment of goat anti-rabbit IgG (H+L) with CF®660C dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes.

CF®583R Annexin V is a photostable deep-red fluorescent conjugate (excitation/emission 583/606 nm) that can be used to stain phosphatidylserine on the surface of apoptotic cells. It is supplied at 50 ug/mL in buffer with azide as a preservative for end-point staining.

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-Red fluorescent CF®594 dye has...