Biotium

5-Aminoallyl-dUTP (AA-dUTP) can be enzymatically incorporated into DNA. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling method. This two-step method for labeling nucleic acids is considerably more...

Goat anti-mouse IgM (μ chain) labeled with CF®555 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange-red fluorescent CF®555 dye has excitation/emission at 555/565...

Biotium's DNA Gel Extraction Kit is a silica-gel spin column based DNA extraction kit designed to purify 40 bp-40 kb DNA fragments from agarose gels in TAE or TBE buffer. Each column has a maximum binding capacity of 10 ug DNA. Biotium's DNA Gel Extraction Kit removes GelRed® and GelGreen® Nucleic...

Dihydroethidium (also called hydroethidium) is the chemically reduced form of the commonly used DNA dye ethidium bromide. The probe is useful to detect oxidative activities in viable cells, including respiratory burst in phagocytes. Dihydroethidium itself has blue fluorescence (λ...

The amine reactive succinimidyl ester of MB (methylene blue) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups. The conjugate will have a blue color and be able to complex with nucleic acids. Methylene blue is a well known redox dye which...

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®660C dye has...

This product is fluorescently-labeled wheat germ agglutinin (WGA). WGA is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine. WGA conjugates can be used to stain glycoproteins that have these sugar moieties. WGA can be used as a gram stain to fluorescently...

Fluorescent conjugates of Alpha-Bungarotoxin bind to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness,...

5-FAM-PEO₈ SE (full name: 5-Carboxyfluorescein-PEO₈ propionate succinimidyl ester) has an approximately 32 Angstrom water-soluble PEG spacer that separates fluorescein and the amine-reactive SE group. &lamba;_Ex/&lamba; _Em(pH9) = 494/521 nm Yellow orange...

CD8a (clone SK1) is a mouse monoclonal antibody that recognizes the human protein CD8. CD8a is a common marker of cytotoxic (Killer) T cells, and is a T cell coreceptor. All conjugates have been validated in flow cytometry. Offered in two sizes: 125uL (25 tests) or 500uL (100 tests). These...

This kit contains the NucView®488 Caspase-3 Substrate and the MitoView™633 mitochondrial dye. The kit provides a convenient tool for profiling apoptotic cells based on caspase-3 activity and changes in the mitochondrial membrane potential using either fluorescence microscopy or flow cytometry. The...

Streptavidin conjugates are prepared from high quality streptavidin biotin binding protein and Biotium’s signature bright and photostable fluorescent CF® dyes, and a selection of other labels.

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. The kits contain PMA dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public...

High-quality goat anti-digoxigenin (DIG) labeled with far-red/near-IR CF®680R dye (excitation/emission 680/701 nm). CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 2mg/mL, 50...

This product is bovine anti-goat IgG labeled with CF®568 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF®568 dye has excitation/emission at 562/583 nm....

This product is prepared by labeling monoclonal mouse anti-biotin with CF®405S dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405S dye has...

TCEP (T ris(2-carboxyethyl)phosphine) hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter. Unlike DTT, TCEP hydrochloride retains its reducing power at acid pH (pH...

Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments.    Dark...

Concentrated buffer stock solution (5X) for one step permeabilization and blocking for immunofluorescence staining. Note: this buffer contains goat serum and therefore is not compatible with anti-goat and anti-sheep secondary antibodies. We recommend Permeabilization Buffer for use with anti-goat...

RNase-X Decontamination Solution effectively removes RNase and other contaminants from working surfaces and instruments. Ideal for use with RNA purification, quantitation and amplification reactions. Performs as well as RNAseZap and other decontamination reagents.

High-quality goat anti-llama IgG (H+L) labeled with far-red fluorescent CF®660R dye (excitation/emission 663/682 nm). CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes.

Neuro-DiO is a derivative of DiO and has spectral properties nearly identical to that of the latter. However, unlike DiO, Neuro-DiO is structurally more similar to DiI, which is known to be a much better lipophilic neuronal tracer than regular DiO. Like DiI in oil, Neuro-DiO is uniformly dissolved...

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. 405 nm-excitable green fluorescent CF®440...

Goat anti-mouse IgG1 isotype-specific secondary antibody labeled with CF®680 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/near-IR fluorescent CF®680 dye has...