Biotium

Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq. The master mix is offered in...

ExoBrite™ CD9 Western Antibody is optimized for detection of the exosome marker CD9 in isolated EV extracts by near-IR (NIR) fluorescent WB. For 25 or 100 mini-blots. • ExoBrite™ Western 680/700 conjugates have excitation/emission at 681/698 nm for detection in the 700 nm channel of near-IR imaging...

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally...

Aminooxy dyes have an aminooxy reactive group, which can be used to label a target molecule with an aldehyde or ketone group. An aminooxy group readily reacts with an aldehyde or ketone group to form a stable linkage without the use of a reducing agent. CF® dyes are Biotium's line of next-generation...

Goat anti-mouse IgG1 isotype-specific secondary antibody labeled with CF®640R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®640R dye has...

Oxazole Red, also known as YO-PRO®-3, is a bright, cell-impermeant, monomeric far-red nucleic acid dye (excitation/emission 612/631 nm). It is essentially nonfluorescent until bound to nucleic acids, making it a useful dead cell stain for microscopy or flow cytometry. 1 mM in DMSO.

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF®800 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared...

Fluorescent conjugates of Alpha-Bungarotoxin bind to the nicotinic acetylcholine receptor with high affinity, and thus can be used to image the distribution of the receptor at the neuromuscular junctions. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness,...

This product is prepared by labeling high quality goat anti-rabbit IgG (H+L) with R-phycoerythrin (R-PE or PE).

Aminooxy reagents such as aminooxy-5(6)-FAM react with aldehydes or ketones to form a stable oxime linkage under mild conditions. Similar to ARP, aminooxy-5(6)-FAM can be used to label abasic sites in damaged DNA. It can also be used to label polysaccharides and glycoproteins....

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF®405M dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF®405M dye has...

This product is prepared by labeling highly cross-adsorbed goat anti-rat IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A...

Cyanine 555-dUTP can be used to synthesize labeled DNA probes for in-situ hybridization, microarray or blotting techniques. Red fluorescent Cyanine 555 is structurally identical to Cy®3 and has excitation/emission at 555/565 nm). Cy dye is a registered trademark of GE Healthcare.

This product is bovine anti-goat IgG labeled with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has excitation/emission at 490/515...

BactoView™ Dead 600/615 is a novel deep-red fluorescent DNA dye for no-wash staining of dead bacteria. Unlike traditional dyes like PI that readily stain live gram+ cells, BactoView™ Dead dyes selectively stain dead cells in both gram+ and gram- strains. 500X in water, 100 uL.

3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or buffer should be used immediately.        Off-white...

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with R-phycoerythrin (R-PE or PE).

Apoptosis and necrosis are two major processes by which cells die. Apoptosis is an active, genetically regulated disassembly of the cell from within. During apoptosis, phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human...

This product is prepared by labeling highly cross-adsorbed donkey anti-rabbit IgG (H+L) with CF®660R dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent red protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety. One-Step Lumitein™ gel staining requires only a single 5-60 minute staining step without fixation....

NBD fluoride (4-Fluoro-7-nitrobenzo-2-oxa-1,3-diazole) is similar to NBD chloride except that the former is much more reactive toward amines. Both reagents are nonfluorescent until they react with primary or secondary amines to produce a fluorescent product. NBD chloride and NBD fluoride have been...

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the...

CF® dye with tetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry. This copper free bioorthogonal reaction is useful for staining the surface of live cells or when there are concerns about native protein function loss with copper in cell...

DASPEI (2-(4-(Dimethylamino)styryl)-N-ethylpyridinium iodide) is used to stain mitochondria in live cells.    &lamba;_Ex/&lamba; _Em = 461/589 nm (MeOH)   Store at 4°C and protect from light, especially in solution   C₁₇H₂₁IN₂...