Biotium

The TrueBlack® Background Suppressor System (Permeabilizing) is a buffer system designed for blocking background from both non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.

Biotin-DHPE (N-(Biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt) can be used to couple avidin or streptavidin to cell membranes or liposomes.    White solid soluble in chloroform   Store at -20°C  ...

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF®488A dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Green fluorescent CF®488A dye has...

Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a K_d of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca 2 but with a significantly lower K_d than Fura-2 for Ca 2...

Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF®633 dye has...

This product is prepared by labeling highly cross-adsorbed rabbit anti-rat IgG (H+L) with CF®594 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent...

CD63 (2304.63) is a recombinant mouse monoclonal antibody that recognizes the human tetraspanin protein CD63. This antibody has been validated in immunofluorescence microscopy. Each is supplied at 5 uL/test, with sufficient antibody for 25 (125uL) or 100 (500uL) tests. These antibodies belong to the...

This product is prepared by labeling high quality goat anti-mouse IgG (H+L) with CF®543 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Orange fluorescent CF®543 dye has...

Goat anti-human IgM (mu chain) is labeled with CF®594 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Deep-red fluorescent CF®594 dye has excitation/emission at 593/614...

High purity resorufin can be used as a standard for enzyme substrates whose end product is resorufin. λ_Ex/λ_Em (pH 9) = 563/587 nm Dark solid soluble in DMSO Store at -20°C and protect from light C₁₂H₇NO₃ MW: 213.04 N

Phosphatidylserine (PS) is translocated from the inner to the outer surface of the cell for phagocytic cell recognition. The human anticoagulant, Annexin V, is a 35 kDa Ca2 -dependent phospholipid protein with a high affinity for PS. Annexin V labeled with CF®488A (Abs/Em= 490/515 nm) can identify...

This product is prepared by labeling highly cross-adsorbed donkey anti-sheep IgG (H+L) with CF®633 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

This product is prepared by labeling highly cross-adsorbed goat anti-human IgG (H+L) with CF®583R dye. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, and mouse serum.

L-NIO ( N 5 -(1-Iminoethyl)-L-ornithine, dihydrochloride) is a potent, irreversible inhibitor of neutrophil nitric oxide synthase. White solid soluble in water Store desiccated at -20°C C₇H₁₇Cl₂N₃O₂ MW: 246.1 [36889-13-1]

The Yeast Viability Staining Kit is used to differentiate live and dead cells in yeast. It has two components: ConA, which stains all cells, and Live-or-Dye™, which stains only dead cells.

This product is prepared by labeling highly cross-adsorbed goat anti-human IgG (H+L) with CF®647 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent...

SynaptoRed™ C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed™ C2 (also called FM4-64) except that the lipophilic tail of SynaptoRed™ C1 is one carbon shorter than that of the SynaptoRed™ C2. Thus, SynaptoRed™ C1 is expected to have a faster off-rate compared to...

SynaptoRed™ C2M is a red fluorescent probe used to follow synaptic vesicles. SynaptoRed™ C2M is slightly more water soluble than SynaptoRed™ C2 (equivalent to FM®4-64). FM is a registered trademark of Thermo Fisher Scientific.

CPM (full name: 7-diethylamino-3-(4'-maleimidylphenyl)-4-methylcoumarin) is probably the most widely used blue fluorescent thiol-reactive dye. This maleimide derivative of coumarin is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation...

This product is prepared by labeling highly cross-adsorbed donkey anti-mouse IgG (H+L) with CF®568 dye. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Red fluorescent CF®568...

The ExoBrite™ Streptavidin Magnetic Beads have high binding capacity and can be used with biotinylated antibodies for efficient isolation of extracellular vesicles (EVs) from biological fluids. They have low background fluorescence for sensitive EV detection by flow cytometry.

BactoSpore™ 488/540 Nucleic Acid Stain is a yellow fluorescent nucleic acid dye that stains live and dead gram-positive and gram-negative bacteria. In B. subtilis, the dye stains both vegetative cells and endospores. excitation/emission 488/536 nm (with DNA). 500X dye in DMSO; 100 uL.

LipidSpot™ 610 dyes rapidly stains lipid droplets with red/far-red fluorescence for detection in the Texas Red® or Cy®5 channels. For live or fixed cells, no wash step required. Note: due to its broad emission, we do not recommend pairing LipidSpot™ 610 with other red or far-red probes.

PMA-PCR kits are designed for selective detection of viable bacteria from a specific strain using PMA dye and real-time PCR. The kits contain PMA dye, Forget-Me-Not™ qPCR Master Mix, and PCR primers for detection of selected strains of bacteria that are of widespread interest to food safety, public...