LGC, Biosearch Technologies

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LGC, Biosearch Technologies

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  • LGC, Biosearch Technologies

    Baseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.

  • LGC, Biosearch Technologies

    The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.

  • LGC, Biosearch Technologies

    Highly cost-effective, direct replacement for AMPure XP beads in PCR and NGS clean-up and size selection applications Affordable: Direct replacement for AMPure XP beads at a fraction of the cost, without sacrificing performance Compatible: Uses same protocol as AMPure XP / SPRIselect …

  • The HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.

  • LGC, Biosearch Technologies

    This reagent is designed for use with the MasterPure™ kit protocols only.

  • LGC, Biosearch Technologies

    Perfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…

  • LGC, Biosearch Technologies

    Lucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…

  • Phage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.

  • LGC, Biosearch Technologies

    EpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT), an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable…

  • LGC, Biosearch Technologies

    Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…

  • LGC, Biosearch Technologies

    Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…

  • LGC, Biosearch Technologies

    Exo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).

  • LGC, Biosearch Technologies

    Lucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…

  • LGC, Biosearch Technologies

    The NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.

  • LGC, Biosearch Technologies

    CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group

  • LGC, Biosearch Technologies

    The LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which…

  • LGC, Biosearch Technologies

    The AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.

  • LGC, Biosearch Technologies

    BAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10^10 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells…

  • LGC, Biosearch Technologies

    Perfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…

  • LGC, Biosearch Technologies

    phi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.

  • LGC, Biosearch Technologies

    RapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…

  • LGC, Biosearch Technologies

    Consistent, high quality beads for single-cell mRNA sequencing applications using Drop-seq1 methods Consistent: Each lot of beads has tightly controlled bead size and produces consistent performance lot to lot Powerful: The 14 base UMI enables sequencing and identification of more unique…

  • LGC, Biosearch Technologies

    The LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…

  • LGC, Biosearch Technologies

    The NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers. Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.

  • LGC, Biosearch Technologies

    RNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.

  • One tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.

  • LGC, Biosearch Technologies

    RNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.

  • LGC, Biosearch Technologies

    Exonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.

  • LGC, Biosearch Technologies

    The MMLV High Performance Reverse Transcriptase produces full-length first-strand cDNA from total RNA preparations or purified poly(A+) RNA.

  • LGC, Biosearch Technologies

    Uracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate

  • LGC, Biosearch Technologies

    The LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

  • LGC, Biosearch Technologies

    RNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi

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