LGC, Biosearch Technologies
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Baseline-ZERO DNase 5,000 U
LGC, Biosearch TechnologiesBaseline-ZERO™ DNase digests dsDNA and ssDNA into mononucleotides more effectively than the commonly used bovine pancreatic DNase I.
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QuickExtract DNA Extraction Solution
LGC, Biosearch TechnologiesThe QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample.
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sbeadex SAB (sequencing application beads)
LGC, Biosearch TechnologiesHighly cost-effective, direct replacement for AMPure XP beads in PCR and NGS clean-up and size selection applications Affordable: Direct replacement for AMPure XP beads at a fraction of the cost, without sacrificing performance Compatible: Uses same protocol as AMPure XP / SPRIselect …
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HI-Control 10G Chemically Competent Cells 12 rxns (SOLOs)
LGC, Biosearch TechnologiesThe HI-Control™ strains are based on E. cloni 10G ( DH10B) and BL21(DE3) strains. These strains harbor a plasmid bearing a constitutive lacI repressor gene, which provides tight control over promoters containing the lacO operator.
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Proteinase K 2 ml
LGC, Biosearch TechnologiesThis reagent is designed for use with the MasterPure™ kit protocols only.
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TG1 Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. The highest efficiency TG1 competent cells available: =4 × 10^10 cfu/µg. TG1 Electrocompetent Cells have an amber suppressor strain (supE) prepared as highly efficient electrocompetent cells for phage display library…
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E. cloni EXPRESS BL21(DE3) Chem Comp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni EXPRESS BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell. Cloning efficiencies are increased 25-1,000 fold relative to competitors’ preparations of BL21 cells, which is essential for construction of…
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TRANSFORMAX EPI300-T1R ELECTRO E. COLI 20 rxns
LGC, Biosearch TechnologiesPhage T1-resistant electrocompetent cells specially engineered to regulate the copy number of CopyControl Fosmid clones.
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EpiScript™ RNase H- Reverse Transcriptase
LGC, Biosearch TechnologiesEpiScript™ Rnase H- Reverse Transcriptase (EpiScript RT), an alternative to SuperScript® II Reverse Transcriptase, is a recombinant MMLV reverse transcriptase with greatly reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. EpiScript RT is capable…
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E. cloni 5-alpha Chemically Competent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™ and directly replace them in cloning protocols. E. cloni chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is…
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E. cloni 10G SUPREME Electrocomp Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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Exo-Minus Klenow DNA Polymerase 1,000 U
LGC, Biosearch TechnologiesExo-Minus Klenow DNA Polymerase is a DNA-dependent DNA polymerase that lacks both the 5´′→3´ and 3´′→5´ exonuclease activities of E. coli DNA Polymerase I, from which it is derived. This N-terminal truncation of DNA Polymerase I has two mutations (D355A and E357A).
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E. cloni 10GF’ Electrocompetent Cells
LGC, Biosearch TechnologiesLucigen’s E. cloni competent cells share the most useful genetic elements of standard cloning strains like DH5a™, DH10B™, JM109, TOP10, etc. and directly replace them in cloning protocols. However, E. cloni electrocompetent cells incorporate a unique manufacturing technology that…
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NxSeq UltraLow DNA Library Kits
LGC, Biosearch TechnologiesThe NxSeq UltraLow DNA Library Kit is used to build DNA fragment libraries for Illumina sequencers. It requires only 50 pg to 75 ng of input DNA. This 96 rxn kit is optimized for library prep in 96-well plates and requires a NxSeq HT Dual Indexing Kit to complete library construction.
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CircLigase II ssDNA Ligase
LGC, Biosearch TechnologiesCircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group
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LavaLAMP™ RNA Master Mix
LGC, Biosearch TechnologiesThe LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which…
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Ampliscribe T7-Flash Transcription Kits
LGC, Biosearch TechnologiesThe AmpliScribe™ T7-Flash™ Transcription Kit produces more RNA in 30 minutes than other in vitro transcription kits produce in two hours.
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BAC-Opt Replicator v2.0 Electrocomp Cells
LGC, Biosearch TechnologiesBAC-Optimized Replicator™ v2.0 Electrocompetent Cells (> 1 × 10^10 cfu/µg pKanR DNA). These cells are required for on-demand, copy number amplification of the pSMART® BAC or pEZ™ BAC vectors in the CopyRight® Kits, thereby increasing DNA yields. BAC-Optimized Replicator Cells…
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SS320 (MC1061 F) Electrocompetent Cells
LGC, Biosearch TechnologiesPerfect for antibody phage display or peptide phage display library creation. SS320 Electrocompetent Cells are a non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (=4 × 10^10 cfu/µg) electrocompetent cells for phage display library screening. These cells have the…
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NxGen phi29 DNA Polymerase
LGC, Biosearch Technologiesphi29 DNA Polymerase is a highly processive DNA polymerase with a powerful strand displacement activity and a 3′→ 5′ proofreading exonuclease function. The enzyme is capable of up to 70,000 insertions per binding event.
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RapiDxFire Thermostable Reverse Transcriptase
LGC, Biosearch TechnologiesRapiDxFire™ Thermostable Reverse Transcriptase is a significant advancement over popular MMLV- and AMV reverse transcriptases. Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10…
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NxSeq Single-cell RNA-seq Beads
LGC, Biosearch TechnologiesConsistent, high quality beads for single-cell mRNA sequencing applications using Drop-seq1 methods Consistent: Each lot of beads has tightly controlled bead size and produces consistent performance lot to lot Powerful: The 14 base UMI enables sequencing and identification of more unique…
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LavaLAMP DNA Component Kits
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit is designed for loop-mediated isothermal amplification (LAMP) assay development. This kit contains a unique, highly processive DNA polymerase enzyme with strong strand displacement activity, making it well suited to LAMP and other isothermal amplification…
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NxSeq Adaptors
LGC, Biosearch TechnologiesThe NxSeq AmpFREE Low DNA Library Kit and Adaptors are only compatible with Illumina sequencers. Each box of NxSeq Adaptors contains (12) different indexed Illumina-compatible adaptors with enough of each adapter for 4 library reactions. Box 1 contains adaptors 1-12 and Box 2 contains adapters 13-24.
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RNase-Free DNase I
LGC, Biosearch TechnologiesRNase-Free DNase I (bovine pancreas) is an endonuclease useful in removing DNA that might interfere with the characterization, manipulation, or use of RNA, or for any application requiring highly purified DNase I, such as nick translation.
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RIBONUCL-5'-TRIPHOSPHATE (NTP) SOL 25 umole ea
LGC, Biosearch TechnologiesOne tube each of ATP, CTP, GTP and UTP at 100 mM. Each is provided as a sterile, neutral solution.
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NxGen RNAse Inhibitor
LGC, Biosearch TechnologiesRNAse Inhibitor is a potent inhibitor of ribonucleases such as RNase A, RNase B, and RNase C. The 52 kDa protein is a fusion of the porcine RNAse Inhibitor gene with a proprietary 22.5 kDa protein tag.
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Exonuclease VII 250 U @ 10 U/µl
LGC, Biosearch TechnologiesExonuclease VII has high enzymatic specificity for ssDNA and exhibits both 5´′→3´ and 3´′→5´ exonuclease activities.
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MMLV HIGH PERFORMANCE REV TRANS 25,000 U
LGC, Biosearch TechnologiesThe MMLV High Performance Reverse Transcriptase produces full-length first-strand cDNA from total RNA preparations or purified poly(A+) RNA.
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Uracil N-Glycosylase 1,000 U
LGC, Biosearch TechnologiesUracil N-Glycosylase (UNG), also known as uracil-DNA glycosylase, hydrolyzes the N-glycosidic bond between the deoxyribose sugar and uracil in DNA containing deoxyuridine in place of thymidine. UNG is active on both ssDNA and dsDNA that contain uracil, but has no activity on RNA or 2´-deoxyuridine- 5´-monophosphate
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Green Fluorescent Dye 500 rxns
LGC, Biosearch TechnologiesThe LavaLAMP™ DNA Component Kit with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.
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RNA 5' Polyphosphatase 200 U
LGC, Biosearch TechnologiesRNA 5´ Polyphosphatase is a Mg2+-independent phosphohydrolase discovered and characterized by Epicentre scientists. The enzyme sequentially removes the ? and ß phosphates from 5´-triphosphorylated RNA (such as primary RNA transcripts): 5´ pppN—OH 3´ ′→ 5´ pN—OH 3´ + 2 Pi
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