Intact Genomics, Inc
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Bsu DNA Polymerase Large Fragment
Intact Genomics, IncBsu DNA Polymerase I, Large Fragment is a product of the Bacillus subtilis DNA polymerase I which lacks the N-terminal exonuclease domain (1-296 amino acids). It retains the 5´→ 3´ polymerase activity of DNA polymerase I but lacks the 5´→ 3´ exonuclease activity.…
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ElectroCompetent Agrobacterium Combo Pack
Intact Genomics, IncIntact Genomics ElectroCompetent Agrobacterium Combo Pack is perfect for scientists who require multiple agrobacterium competent cell strains for their research. The ElectroCompetent Agrobacterium Combo Pack contains 3 50µl aliquots of GV3101, AGL1, EHA105 and LBA4404 along with pCambia…
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Taq DNA Polymerase
Intact Genomics, IncIntact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration…
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DH10B Chemically Competent Cells (ig™10B)
Intact Genomics, IncIntact Genomics ig™ 10B chemically competent cells (E. coli) are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. This derivative of DH10B provides the highest efficiency in the industry. Product Includes: …
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igScript™ Probe Based RT-qPCR 2x master mix
Intact Genomics, IncIgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior sensitivity and specificity. IgScript™ qPCR 2x…
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DH10B Electrocompetent Cells (ig™10B)
Intact Genomics, IncIntact Genomics ig™ 10B (DH10B derivative) electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 1010 cfu/µg plasmid DNA which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. …
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DirectPlate™ 10B Chemically Competent Cells
Intact Genomics, IncIntact Genomics (ig®) DirectPlate™ Competent cells offer simple, fast, and robust results for your DNA transformation needs. DirectPlate™ 10B chemically competent E. coli cells are a perfect choice for researchers looking to simplify their transformation workflow by eliminating heat…
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i7™ Hot Start High-Fidelity DNA Polymerase
Intact Genomics, Inci7™ Hot Start High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10^2 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…
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BL21(DE3) Electrocompetent Cells
Intact Genomics, IncIntact Genomics BL21 (DE3) electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21(DE3) electrocompetent E. coli cells ideal for construction of complex expression…
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LBA4404 ElectroCompetent Agrobacterium
Intact Genomics, IncIntact Genomics LBA4404 ElectroComp Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of…
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ig SYBR Green qPCR 2X Master Mix
Intact Genomics, IncIntact Genomics SYBR Green qPCR 2X Master Mix master mix is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The Ig SYBR® Green qPCR 2x master mix with integrated chemically-modified hot start Taq DNA polymerase,…
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igMax™ DH10B ElectroCompetent Cells
Intact Genomics, IncIntact Genomics igMax™ DH10B derivative ElectroCompetent cells are suitable for demanding cloning situations, such as synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets, requiring the greatest…
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T4 DNA Polymerase
Intact Genomics, IncIntact Genomics T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. Product Includes: T4 DNA Polymerase 10x T4 DNA Polymerase Buffer
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Cas9 Nuclease
Intact Genomics, IncClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids (1). The CRISPR system consists of a…
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T4 gp32 Protein
Intact Genomics, IncT4 gp32 Protein is a single-stranded DNA binding protein required for T4 DNA replication, recombination, and repair (1, 2). It improves the efficiency of reverse transcriptase (RT) during RT-PCR (3), enhances T4 DNA polymerase activity (4), as well as increases the yield of PCR products (5). …
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igScript™ Reverse Transcriptase
Intact Genomics, IncigScript™ Reverse Transcriptase (igScript RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. igScript RT is an RNA-directed DNA polymerase which lacks…
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igScript™ RT-PCR kit
Intact Genomics, IncigScript™ RT-PCR Kit combines two powerful mixtures: i). 5x igScript™ master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.c Product…
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igScript™ Probe Based RT-qPCR Kit
Intact Genomics, IncIgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures…
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Hot Start Taq DNA Polymerase
Intact Genomics, IncIntact Genomics Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the…
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GV3101 Electrocompetent Agrobacterium
Intact Genomics, IncGV3101 strain (A. tumefaciens) has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results…
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T5 Exonuclease
Intact Genomics, IncT5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA (1). The mode of action of T5 Exonuclease in vivo may be…
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igScript™ One Step RT-PCR Kit
Intact Genomics, IncigScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction…
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ig-Fusion™ Cloning Kit
Intact Genomics, IncIntact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase…
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T4 UvsY Protein
Intact Genomics, IncUvsY is the phage T4 recombination mediator protein, and structural and biophysical studies provide insights into its role in T4 homologous recombination. During T4 homologous recombination, the UvsX recombinase must compete with the prebound gp32 single-stranded binding protein for DNA-binding…
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DL39 (DE3) Chemically Competent Cells
Intact Genomics, IncIntact Genomics chemically competent DL39 (DE3) E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label :e.g. phenylalanine or leucine residues. DL39 (DE3) can also be used to reduce NMR cross-labeling via transaminase activity for…
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T4 Polynucleotide Kinase (PNK)
Intact Genomics, IncT4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes…
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i7™ High-Fidelity DNA Polymerase
Intact Genomics, Inci7™ High-Fidelity DNA Polymerase has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes. Applications: Long and difficult template DNA…
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AGL1 Electrocompetent Agrobacterium
Intact Genomics, IncThe AGL1 strain (A. tumefaciens) has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicilin resistance in its genome for selection. AGL1 contains the Ti plasmid pTiBO542…
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ig™ 5-alpha Electrocompetent Cells
Intact Genomics, IncIntact Genomics 5-alpha electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality. …
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One Step RT-qPCR kit for SARSCoV-2
Intact Genomics, IncFor research use only. Not to be used for diagnostic purposes. Intact Genomics COVID-19 or SARS-CoV-2 Coronavirus detection kit is used for in vitro detection of SARS-CoV-2 using Real-Time quantitative PCR (RT-qPCR). The coronavirus SARS-CoV-2 was announced as the etiological agent of…
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igScript™ One Step RT-qPCR Kit
Intact Genomics, IncigScript™ one step RT-qPCR kit combines two powerful mixtures: i). igScript™ Reverse Transcriptase and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require…
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igScript™ Probe Based RT-qPCR Kit
Intact Genomics, IncIgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures…
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