Western Blotting and ELISA Supplies
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Western Blotting Boxes
MTC BioNo matter what size your Western blot is, there’s a box that fits it perfectly. With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. Additionally, all corners are formed at…
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WesternBright™ Sirius™ HRP Substrate
AdvanstaSee bands you couldn't detect before WesternBright Sirius is the most sensitive chemiluminescent substrate available for Western blotting. Its extremely strong signal allows detection of bands unseen with other substrates. Features The most sensitive of Advansta's HRP…
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0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…
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2.45mm thickness, replaces paper towels Completes blotting "sandwich" Smoother, more uniform texture
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Anti-MOUSE IgG (H&L) (GOAT) Antibody DyLight™
Rockland Immunochemicals IncThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™…
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100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…
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HyBlot 26™ Blotting Paper, Dimensions: 14 x 17 in. (35.5 x 43cm), Qty: 100 0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches
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Incubation Trays
Azure BiosystemsTray with clear top and bottom for staining and washing gels and membranes. Tray with opaque black top and bottom for staining and washing light sensitive gels and membranes.
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0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.
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0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.
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LucentBlue™ X-Ray Film
AdvanstaHigh sensitivity X-ray film for chemiluminescent Western blots LucentBlue is a sensitive film ideal for imaging chemiluminescent Western blots. LucentBlue is guaranteed to perform with WesternBright™ ECL, WesternBright Quantum and WesternBright Sirius. LucentBlue is compatible with…
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0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches
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STREPTAVIDIN ATTO
Rockland Immunochemicals IncThe emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
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0.18mm thickness Medium flow rate Compare to 1Chr Ideal for use in paper chromatography or as a gel dryer support. Also suitable for use as a spacer in blotting sandwiches
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F(ab')2 Anti-FLUORESCEIN (GOAT) Antibody
Rockland Immunochemicals IncThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms as well as other antibody based…
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Pre-Cut Blotting Papers
NuSepNuSep’s Pre-cut blotting papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. Convenient – pre-cut; works with most mini gel transfer assemblies Tested –…
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HEK293 Whole Cell Lysate, 500µg, Liquid
Rockland Immunochemicals IncReady-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…
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WesternBright™ ChemiPen™
AdvanstaTransform your protein standard into a chemiluminescent marker Write or draw on your transfer membranes with the WesternBright ChemiPen. With the proprietary "ink" you can make pre-stained protein standards chemiluminescent, annotate your blot with a date or blot number, or check…
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BOVINE SERUM ALBUMIN 30% Solution
Rockland Immunochemicals IncBOVINE SERUM ALBUMIN 30% Solution is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody, and use as a blocking agent to reduce non-specific binding.
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0.40mm thickness Compare to 3Chr Commonly used as wicks in protein or nucleic acid blotting. Provides uniform buffer flow through gel to transfer membrane.
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Anti-GUINEA PIG IgG (H&L) (GOAT) Antibody
Rockland Immunochemicals IncThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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PROTEIN G PEROXIDASE Conjugated, 500µg, Lyophilized
Rockland Immunochemicals IncProtein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein G…
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100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is manufactured…
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WesternBright™ Transfer Membranes
AdvanstaConvenient Nitrocellulose and PVDF membranes for Western blotting Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical…
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STREPTAVIDIN DyLight™
Rockland Immunochemicals IncThis product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™…
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F(ab')2 Anti-CAT IgG [H&L] (RABBIT) Antibody
Rockland Immunochemicals IncSecondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods.
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100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is manufactured and…
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Blotting Papers
AdvanstaAdvansta's pre-cut Blotting Papers are ideal for use in transfer sandwiches and cassettes when proteins are transferred electrophoretically from a gel to a nitrocellulose, PVDF, or other membrane. The Advansta Blotting Papers are available in two sizes to work with multiple transfer setups and…
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Human Foreskin Fibroblast Whole Cell Lysate, 500µg, Liquid (sterile filtered)
Rockland Immunochemicals IncReady-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes…
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Blotting Paper for Western Blot
Azure BiosystemsBlotting Paper for Western Blot (7x9) Pre-cut blotting paper for Western blot transfer (7x9 cm vertical gels); 60 sheets Blotting Paper for Western Blot (8x10) Pre-cut blotting paper for Western blot transfer (8x10 cm vertical gels); 60 sheets
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BOVINE SERUM ALBUMIN
Rockland Immunochemicals IncBovine Serum Albumin - Fraction V is suitable for use in protease sensitive assays such as RIA, EIA and nucleic acid hybridization, use as a stabilizing agent for proteins and enzymes including dilute solutions of antibody, use as a blocking agent to reduce non-specific binding for FACS, IF, IHC,…
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WESTERN INCUBATION BOX, LARGE (4 5/8'' X 3 1/2'' X 1 1/8'') , 5-PACK, 1Each
Rockland Immunochemicals IncBlack plastic hinged box ideal for the incubation, blocking, and washing steps for Western Blotting using Fluorescent Conjugated Antibodies such as DyLight Conjugated Secondary Antibodies or IRDye Antibody Conjugates. This large box is best for volumes between 10mL and 30mL.
