Western Blotting and ELISA Chemicals
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femtoLUCENT™ PLUS AP Kit
G-BiosciencesThe femtoLUCENT™ PLUS-AP kit is based on alkaline phosphatase (AP) chemiluminescence reaction. The chemiluminescence light emission can be recorded by a short exposure to blue-light sensitive autoradiography films. The femtoLUCENT™ PLUS-AP kit allows for detection of femtogram quantity…
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TBST Buffer 10x Strength Solution
Bio Basic Inc.TBST buffer 10x Strength Solution: Ultra Pure 100mM Tris, 1.5M Sodium Chloride, 0.5% Tween-20 Supplied as a 10X stock solution, when diluted to 1X pH should be around 7.4 and 0.2μm filtered.
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femtoELISA™
G-BiosciencesEnzyme-Linked Immunosorbent Assay (ELISA) is a useful, highly sensitive and powerful technique for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine. The key to an ELISA is the interaction of a known antibody with the antigen of…
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0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…
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NAP-BLOCKER™
G-BiosciencesA major drawback of current blocking solutions, such as BSA , casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the…
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BLOK™ BSA
G-BiosciencesProtein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …
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Blot Boxes
MTC BioMinimize antibody usage Hinged lid reduces evaporation For halves, wholes, strips, or minis Whether you're doing Western blots in halves, wholes, strips or minis, we have the right Western blot box for you. Choose the size that suits your needs and you'll be able to instantly reduce…
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Blocking Solution
Quality BiologicalBlocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…
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Enhancer™ Dot Blot System
G-BiosciencesEnhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion. …
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Well-Coated™ Glutathione Plates
G-BiosciencesWell-Coated™ Glutathione plates are designed to specifically bind GST (Glutathione S-Transferase) tagged proteins and peptides. The plates have immobilized glutathione and can be used for the isolation of GST-tagged proteins direct from bacterial lysates and then used for subsequent ELISA…
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picoLUCENT™ PLUS-HRP Kit
G-BiosciencespicoLUCENT™ PLUS-HRP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-HRP reagents are…
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FLASHBlot™ Transfer Buffers
AdvanstaEnhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…
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BLOK™ BLOTTO (5% modified milk protein solution)
G-BiosciencesConvenient ready-to-use blocking solution for Western blots, dot Blots & ELISA Features 5% novel modified milk protein solution Use for Westerns, ELISA, and dot Blot blocking Blocking in 10-15 minutes Ready-to-use
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Swift Membrane Stain™
G-BiosciencesSwift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and…
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Nitrocellulose Transfer Membrane 0.22um (8x10)
Azure BiosystemsPre-cut membrane (8x10 cm mini vertical gels, ideal for smaller proteins); 10 sheets
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Nitrocellulose Membranes
G-BiosciencesFor user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. …
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AzureRed Fluorescent Protein Stain
Azure BiosystemsFluorescent total protein stain for 1D and 2D gels – sufficient for 20 minigels or 40 miniblots; 5 ml
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Well-Coated™ Sulfhydryl Binding Plates
G-BiosciencesWell-Coated ™ Sulfhydryl Binding plates are designed to specifically bind free sulfhydryls of peptides, proteins and other molecules. The Well-Coated ™ Sulfhydryl Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other…
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picoLUCENT™ PLUS AP Kit
G-BiosciencespicoLUCENT™ PLUS-AP kit is based on our ultra sensitive Luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films. picoLUCENT™ PLUS-AP is also…
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Avant™ Buffer Pouches
AdvanstaConvenient, pure, fast buffer preparation Advansta's Avant buffer pouches are perfect for standardizing your electrophoresis and Western blotting applications. Dissolve a pouch of pre-measured molecular biology-grade chemicals in 500mL of deionized water to obtain a ready-to-use 1x…
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Radiance Plus
Azure BiosystemsFemtogram HRP substrate (150 ml kit); contains substrate for 1,500 cm2 membrane
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Well-Coated™ Protein A Plates
G-BiosciencesWell-Coated ™ Protein A plates are designed to bind the constant (Fc) region of immunoglobulins ensuring that the antigen binding domain of the antibody is orientated away from the plate, offering maximum exposure of the binding site. The immunoglobulin orientation on the Well-Coated ™…
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AzureSpectra Labeling Kit – for Western Blot – 490
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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AzureSpectra Labeling Kit – for Western Blot – 800
Azure BiosystemsKit for covalently labeling antibodies for Western blot with a fluorescent dye – includes labeling buffer, dye solution, quenching solution, neutralization buffer and two G-25 columns – each kit includes sufficient reagents to perform two labelings of 50 micrograms of antibody
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AzureSpectra IR ms700/rb800 Western Kit, with Fluorescent Block
Azure BiosystemsKit contains materials for 10 Western blots – LF PVDF membranes, wash buffer, fluorescent blocking buffer, secondary antibodies AzureSpectra ms700/rb800
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AzurePonceau Stain
Azure BiosystemsVisible reversible total protein stain for proteins transferred to membrane – sufficient for staining 50 blots 9x7 cm; 500 ml
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FISH-Blocker
G-BiosciencesFISH-Blocker is a blocking agent that uses a fish protein as the primary blocking agent. The use of a fish protein, a non-mammalian protein, is that it eliminates or minimizes the interaction of antibodies raised in mammals. FISH-Blocker is one of the best blocking agents for immunoassays and it…
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4CN Stain Kit
Bio Basic Inc.The Bio Basic Inc. 4CN stain kit is suitable for laboratory and research use. It is mainly used in the studies and analysis related to RNA and DNA.
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AdvanBlock™-PF Blocking Solution
AdvanstaNon-protein blocking solution for fluorescent and chemiluminescent Western blotting AdvanBlock-PF is a protein-free blocking solution, optimized for use with the WesternBright MCF fluorescent Western blotting kit and also an excellent blocking reagent for chemiluminescent Western blotting.…
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Well-Coated™ Neutravidin Plates
G-BiosciencesWell-Coated ™ Neutravidin™ plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies, with minimal non-specific binding. This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin…
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Adalimumab Elisa Kit
Lab BioreagentsAdalimumab ELISA Kit is a sandwich enzyme-linked immunoassay for the quantitative determination of Adaliumab with high sensitivity and specificity in serum and plasma. Kit Performance Sensitivity: 25 ng/mL Assay Range: 25 – 2000 ng/mL Cross-reactivity: There is no cross reaction with…
