A working concentration of 500 µg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels. For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of…

Crude Phaseolus vulgaris-P lectin (PHA-P) from the Red Kidney Bean. Phytohemagglutinin PHA agglutinates the erythrocytes of all human blood groups as well as those of rabbit, dog, cat, mouse, sheep, and guinea pig. The optimal amount of PHA required for the agglutination of erythrocytes varies with…

The human PDE6B-6*his fusion protein from Proteintech is 1 - 88 aa of human PDE6B expressed in E. coli with the expression vector PET28a. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

Gel Shift/Gel Super Shift Assays: the electrophoretic gel shift assay is used to detect sequence specific DNA-binding proteins present in nuclear extracts. For NFkB generally a HeLa nuclear extract is used. In the assay, a consensus oligonucleotide is end-labeled with isotopic phosphorus and…

An intact toxin that catalyzes the ADP-ribosylation and inactivation of EF-2, terminating protein synthesis and causing cell death.

The human CCDC8-gst fusion protein from Proteintech is 60 - 196 aa of human CCDC8 expressed in E. coli with the expression vector PGEX-4T. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

Casein hydrolysate enzymatic is a pancreatic hydrolysate of casein. This hydrolysate contains, in the form of mixed amino acids and peptides, all amino acids originally present in casein. It is a refined product providing high quality soluble source of amino acids, polypeptides and peptides. …

Recombinant streptavidin from streptomyces avidinii, produced in E.coli. Biotin, a 244Da vitamin (Vitamin H) molecule, exhibits an extraordinary binding affinity for streptavidin (Ka=10 15 M -1 ). Biotin and streptavidin interaction is rapid and once the bond is established it can survive up…

Swine IgG Whole Molecule Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.

The total protein is determined using the Biuret procedure, with bovine albumin as the standard. Each vial is overfilled by approximately 10 percent so that a minimum of 20 mg of mouse IgG is obtained upon reconstitution. Mouse IgG is purified from pooled mouse serum using multi-step…

Dog IgG whole molecule can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

The human AKR1B1-6*his fusion protein from Proteintech is 1 - 316 aa of human AKR1B1 expressed in E. coli with the expression vector PET28a. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

The human SQSTM1-gst fusion protein from Proteintech is 1 - 440 aa of human P62;SQSTM1 expressed in E. coli with the expression vector PGEX-4T. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

LL-37, K-TAMRA Control Peptide is suitable for use in ELISA, Western Blot, Dot blot, PCA, and other assays. Control peptide should be used at 1.0 µg per 1.0 µl of antiserum in per assay. Specific conditions for reactivity should be optimized by the end user.

Casein is the name for a family of related phosphoproteins (αS1, αS2, β, κ). These proteins are commonly found in mammalian milk, making up 80% of the proteins in cow milk and between 20% and 45% of the proteins in human milk. Casein has a wide variety of uses, from being a…

Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins. Canine…

Application Cell adhesion assays. Optimal working dilutions must be determined by end user. General description Product Source: Human glioma cell line (U251). Purified Human Tenascin-C Protien. Tenascin is known to: 1) play an active role in development of central nervous…

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Glycoprotein which is a highly active thiol proteinase Non-specific cysteine protease Splits glycine esters but not lysine esters Protein digesting…

The human IFITM3-6*his fusion protein from Proteintech is 1 - 133 aa of human IFITM3 expressed in E. coli with the expression vector PET28a. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

Yeast Hydrolysate Enzymatic is an autolyzed yeast extract produced from 100% brewers yeast. It is a concentrated source of soluble protein components, carbohydrates, and B Complex vitamins natural to yeast.

Source: one ampoule contains 100 µg of recombinant truncated human thioredoxin

The human VPS4A-gst fusion protein from Proteintech is 86 - 437 aa of human VPS4A expressed in E. coli with the expression vector PGEX-4T. This protein has been detected in SDS-page gel. The protein is lyophilized powder.

Extract-EZ E1, Total Subcellular Protein Extraction Kit: The kit is designed for extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/organelle, and cytoskeleton protein) from adherent and suspension cultured mammalian cells. The procedure is fast and simple. The whole…

TRDMT1 Protein is stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. TRDMT1 Protein is suitable for use in Western Blot. Expect a band approximately ~67kDa on specific lysates or tissues. Specific conditions for reactivity should be…

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the…

Gum Arabic can be branched polymer of galactose, rhamnose, arabinose, and glucuronic acid as the calcium, magnesium, and potassium salts. It is used in textiles, ceramics, lithography, cosmetics and pharmaceuticals. In the food industry, acacia is mainly used as confectionery, bakery, dairy,…