FirstChoice ™ Blocking Buffers contain a proprietary protein formulation that offers greater versatility and lack of cross-reactivity. This makes FirstChoice ™ Blocking Buffers ideal as a first choice for optimization of new assays, system or when determining the optimal blocking…

10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum…

Superior ™ Blocking Buffer contains a proprietary antiginically non-determinant protein for blocking non-specific sites during ELISA, membrane blotting, immunohistochemistry and other applications. Superior ™ Blocking Buffer is ideal for a high signal to background ratio in most…

Well-Coated™ Glutathione plates are designed to specifically bind GST (Glutathione S-Transferase) tagged proteins and peptides. The plates have immobilized glutathione and can be used for the isolation of GST-tagged proteins direct from bacterial lysates and then used for subsequent ELISA…

Chemiluminescent substrate for ELISA applications ELISABright is an enhanced and highly sensitive chemiluminescent HRP substrate optimized for chemiluminescent ELISA applications. This luminol-based substrate demonstrates a wide linear dynamic range and a superior signal to noise ratio.…

0.83mm thickness, smooth surface Economical - compare to GB003 Generally used as a blotter under the "weight" of a capillary sandwich. Assures continuous contact of buffer from the chamber through the gel and transfer membrane to the opposite pole (electroblot) or weight…

100% pure cotton fiber; 0.345mm thickness Sheets are smooth, flat and RNase free Economical - compare to 3MM Our HyBlot 3A™ paper is specifically designed, controlled and packaged for electrophoresis and blotting applications. High purity grade cotton fiber paper is…

Ahlstrom manufactures a complete line of high quality chromatography, electrophoresis and blotting papers for use in gel transfer applications and chromatography techniques. These uniform, smooth, white sheets are the high purity, high quality, and high value standard. They are used in nucleic acid…

Well-Coated ™ Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. The Well-Coated ™ Amine Binding plates are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for…

Transparent plastic sheets to hold Western blots for imaging – alternative to plastic wraps; 100 pcs

Western blot analysis of proteins is a routine and commonly used technique in research laboratories, with 3 major drawbacks. The first is the amount of time taken to transfer the proteins to a protein binding membrane; the second is the variable efficiency of the transfer and the third is problems…

Swift™ Membrane Stain is a unique, proprietary (patents pending), rapid, reversible, ready-to-use membrane stain for proteins on nitrocellulose or PVDF membranes. Swift™ Membrane Stain stains proteins faster and with 500 times more sensitivity than the routinely used Ponceau-S stain and…

Well-Coated ™ Streptavidin plates are designed to specifically bind biotinylated molecules, including biotin tagged antibodies.This is particular advantageous for antibodies known to denature upon direct binding to polystyrene plates. Biotin exhibits an extraordinary binding affinity for…

For user's convenience, we offer pre-cut transfer membranes and padding for Western blot transfer procedures. Pre-cut membranes are supplied sandwiched between blotting paper padding. Simply soak the membrane in transfer buffer and assemble with the gel in a transfer cassette. …

Pre-mixed, standard Laemmli loading buffers for protein electrophoresis These ready-to-use Laemmli loading buffers contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis. Choose from reducing or non-reducing buffers, each supplied…

Well-Coated™ Nickel plates are designed to specifically bind 6X histidine (polyhistidine) tagged proteins and peptides. The plates can be used for the isolation of polyhistidine-tagged proteins direct from bacterial lysates and then used for subsequent ELISA protocols. The wells are…

Enhancer™ allows for the rapid application of up to 384 protein or nucleic acid samples on to a 12 x 9cm membrane for subsequent analysis. Samples are applied to a nitrocellulose or nylon membrane by capillary action, resulting in concentration of sample due to minimal diffusion. …

Enzyme-Linked Immunosorbent Assay (ELISA) is a useful, highly sensitive and powerful technique for the detection of proteins, chemicals, and drugs (antigens) in biological samples, including serum, blood and urine. The key to an ELISA is the interaction of a known antibody with the antigen of…

Enhanced protein transfer for improved sensitivity Achieve improved detection of low-abundance and posttranslationally modified proteins with Advansta's proprietary FLASHBlot Transfer Buffer. High-efficiency protein transfer and increased protein retention on the membrane add up to more…

Washing solutions optimized for use with WesternBright systems AdvanWash washing solution is optimized to work with Advansta Western blotting system. Choose AdvanWash for all WesternBright chemiluminescent HRP substrates and the WesternBright MCF fluorescent Western blotting kit.…

AdvanStain Scarlet is a fluorescent stain for gels and blots that allows sensitive and quantitative visualization of proteins. Gels and blots stained with AdvanStain Scarlet can be imaged on any fluorescence imaging system, including laser- and CCD-based systems, with a detection limit of less than…

A major drawback of current blocking solutions, such as BSA , casein and milk powder, is that they are derived from animal sources. The presence of animal proteins can often lead to high non-specific backgrounds as antigens and antibodies, generated in animals, interact with the…

Protein-Free Blocking Buffer does not contain protein; it is a proprietary formation of non-protein agents that eliminates non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free Blocking Buffer eliminates problems…

Uniformly removes antibodies from developed HRP Western blots so the blot can be reprobed with another set of antibodies; 500 ml

Minimize antibody usage Hinged lid reduces evaporation For halves, wholes, strips, or minis Whether you're doing Western blots in halves, wholes, strips or minis, we have the right Western blot box for you. Choose the size that suits your needs and you'll be able to instantly reduce…

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Reduce nonspecific binding Applications Quality Biological’s Blocking Solution is prepared from non-fat dried milk in PBS containing sodium azide 0.02% (w/v) as a…

This medium thick paper offers extremely high flow and is recommended for both electrophoresis of large molecules and as a blotter to complete the blotting sandwich in gel transfer applications. The smooth and uniform texture assures continuous contact of buffer from the chamber through the gel and…

This super thick grade is one of the thickest papers on the market, and offers extremely high flow. It is recommended both for electrophoresis of large molecules and as a blotter to complete the blotting sandwich in gel transfer applications. This blotting paper provides a smooth and uniform…

Convenient, easy to use trays for staining and washing gels and membranes These specially designed incubation trays are ideal for staining and washing electrophoresis gels and membranes. Chose from three designs, all available in a range of sizes: Opaque – for light-sensitive…

Fluorescent total protein stain for 1D and 2D gels – sufficient for 20 minigels or 40 miniblots; 5 ml

Protein solutions are used for blocking excess binding sites in ELISA, blotting, immunohistochemistry and other immunochemical applications. Each blocking solution will impart certain characteristics on the assay system. Our BLOK™ BSA is a 10% solution of BSA (Bovine Serum Albumin) …

TBST buffer 10x Strength Solution: Ultra Pure 100mM Tris, 1.5M Sodium Chloride, 0.5% Tween-20 Supplied as a 10X stock solution, when diluted to 1X pH should be around 7.4 and 0.2μm filtered.