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Material Safety Data Sheet
Section 1. Product and Company Identification
Product Name Hemacolor® Product Code 65044
Manufacturer
EMD Chemicals Inc.
P.O. Box 70
480 Democrat Road
Gibbstown, NJ 08027
Prior to January 1, 2003 EMD Chemicals Inc. was EM Industries, Inc. or EM Science, Division of EM Industries, Inc.
Effective Date
4/19/2005
For More Information Call In Case of Emergency Call
856-423-6300 Technical Service
Monday-Friday: 8:00 AM - 5:00 PM		 800-424-9300 CHEMTREC (USA)
613-996-6666 CANUTEC (Canada)
24 Hours/Day: 7 Days/Week
Synonym
Solution 1, 65044A, Fixative Methyl Alcohol
Hemacolor Solution 2, Fixative None.
HARLECO® Hemacolor® Solution 3 None.
Material Uses
Solution 1, 65044A, Fixative Analytical reagent.
Hemacolor Solution 2, Fixative Laboratory Reagent
HARLECO® Hemacolor® Solution 3 Laboratory Reagent
Chemical Family
Solution 1, 65044A, Fixative Alcohol.
Hemacolor Solution 2, Fixative Solution.
HARLECO® Hemacolor® Solution 3 Solution.

Section 2. Composition and Information on Ingredients
Product name / Reagent. Component ; CAS # ; % by Weight
Solution 1, 65044A, Fixative Methanol; 67-56-1; 100%
Hemacolor Solution 2, Fixative Water; 7732-18-5; 97.97%
Potassium Phosphate, Monobasic; 7778-77-0; 0.9%
Sodium Azide; 26628-22-8; 0.1%
Sodium Phosphate, Dibasic ; 7558-79-4; 0.9%
Eosin Y; 17372-87-1; 0.9%
HARLECO® Hemacolor® Solution 3 Water; 7732-18-5; 96.96%
Potassium Phosphate, Monobasic; 7778-77-0; 0.9%
Sodium Phosphate, Dibasic ; 7558-79-4; 0.9%
Methylene Blue; 61-73-4; 0.9%
Azure B; 531-55-5; 0.9%

Section 3. Hazards Identification
Physical State and Appearance
Solution 1, 65044A, Fixative Liquid. (Colorless.)
Hemacolor Solution 2, Fixative Liquid.
HARLECO® Hemacolor® Solution 3 Liquid.
Emergency Overview
Solution 1, 65044A, Fixative MAY BE FATAL IF SWALLOWED.
HARMFUL IF INHALED OR ABSORBED THROUGH SKIN.
CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION.
CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: GASTROINTESTINAL TRACT, RESPIRATORY TRACT, SKIN, EYES, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA.
FLAMMABLE LIQUID AND VAPOR.
VAPOR MAY CAUSE FLASH FIRE.
Hemacolor Solution 2, Fixative CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, MUCOUS MEMBRANES, SKIN, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA.
MAY BE HARMFUL IF INHALED, ABSORBED THROUGH SKIN OR SWALLOWED.
MAY CAUSE EYE AND SKIN IRRITATION.
HARLECO® Hemacolor® Solution 3 CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: MUCOUS MEMBRANES, SKIN, EYES.
MAY CAUSE EYE AND SKIN IRRITATION.
Solution 1, 65044A, Fixative Not Listed on Ca Prop 65.
Hemacolor Solution 2, Fixative Not Listed on Ca Prop 65.
HARLECO® Hemacolor® Solution 3 Not Listed on Ca Prop 65.
Routes of Entry
Solution 1, 65044A, Fixative Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.
Hemacolor Solution 2, Fixative Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.
HARLECO® Hemacolor® Solution 3 Dermal contact. Eye contact. Inhalation. Ingestion.
Potential Acute Health Effects
Eyes
Solution 1, 65044A, Fixative Hazardous in case of eye contact (irritant). Inflammation of the eye is characterized by redness, watering, and itching.
Hemacolor Solution 2, Fixative May be hazardous in case of eye contact (irritant).
HARLECO® Hemacolor® Solution 3 May be hazardous in case of eye contact (irritant).
Skin
Solution 1, 65044A, Fixative Hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
Hemacolor Solution 2, Fixative May be hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
HARLECO® Hemacolor® Solution 3 May be hazardous in case of skin contact (irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
Inhalation
Solution 1, 65044A, Fixative Hazardous in case of inhalation (lung irritant).
Hemacolor Solution 2, Fixative May be hazardous in case of inhalation.
HARLECO® Hemacolor® Solution 3 No known acute effects of this product resulting from inhalation.
Ingestion
Solution 1, 65044A, Fixative Extremely hazardous in case of ingestion. May be fatal if swallowed.
Hemacolor Solution 2, Fixative May be hazardous in case of ingestion.
HARLECO® Hemacolor® Solution 3 No known acute effects of this product resulting from ingestion.
Potential Chronic Health Effects
Carcinogenic Effects
Solution 1, 65044A, Fixative This material is not known to cause cancer in animals or humans.
Hemacolor Solution 2, Fixative This material is not known to cause cancer in animals or humans.
HARLECO® Hemacolor® Solution 3 This material is not known to cause cancer in animals or humans.
Additional information See Toxicological Information (section 11)
Medical Conditions Aggravated by Overexposure:
Solution 1, 65044A, Fixative Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs.
Hemacolor Solution 2, Fixative Repeated or prolonged exposure is not known to aggravate medical condition.
HARLECO® Hemacolor® Solution 3 Repeated or prolonged exposure is not known to aggravate medical condition.

Section 4. First Aid Measures
Eye Contact
Solution 1, 65044A, Fixative Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention immediately.
Hemacolor Solution 2, Fixative Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention.
HARLECO® Hemacolor® Solution 3 Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention.
Skin Contact
Solution 1, 65044A, Fixative In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Cover the irritated skin with an emollient. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Hemacolor Solution 2, Fixative In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove contaminated clothing and shoes. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention.
HARLECO® Hemacolor® Solution 3 In case of contact, immediately flush skin with plenty of water. Cover the irritated skin with an emollient. Remove contaminated clothing and shoes. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention.
Inhalation
Solution 1, 65044A, Fixative If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately.
Hemacolor Solution 2, Fixative If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention.
HARLECO® Hemacolor® Solution 3 If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention.
Ingestion
Solution 1, 65044A, Fixative If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately.
Hemacolor Solution 2, Fixative Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband.
HARLECO® Hemacolor® Solution 3 Do NOT induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. If large quantities of this material are swallowed, call a physician immediately. Loosen tight clothing such as a collar, tie, belt or waistband.

Section 5. Fire Fighting Measures
Flammability of the Product
Solution 1, 65044A, Fixative Product will burn.
Hemacolor Solution 2, Fixative Non-flammable.
HARLECO® Hemacolor® Solution 3 Non-flammable.
Auto-ignition Temperature
Solution 1, 65044A, Fixative 464.05°C (867.3°F)
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Flash Points
Solution 1, 65044A, Fixative Open cup: 15.9°C (60.6°F).
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Flammable Limits
Solution 1, 65044A, Fixative LOWER: 6% UPPER: 36.5%
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Products of Combustion
Solution 1, 65044A, Fixative These products are carbon oxides (CO, CO2).
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Fire Hazards in Presence of Various Substances
Solution 1, 65044A, Fixative Highly flammable in presence of open flames, sparks and static discharge, of shocks, of heat, of oxidizing materials.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Explosion Hazards in Presence of Various Substances
Solution 1, 65044A, Fixative Risks of explosion of the product in presence of static discharge:
Highly flammable in presence of open flames, sparks and static discharge.
Highly explosive in presence of open flames, sparks and static discharge.

Risks of explosion of the product in presence of mechanical impact:
Highly flammable in presence of shocks.
Highly explosive in presence of shocks.
Hemacolor Solution 2, Fixative Risks of explosion of the product in presence of static discharge: No.

Risks of explosion of the product in presence of mechanical impact: No.
HARLECO® Hemacolor® Solution 3 Risks of explosion of the product in presence of static discharge: No.

Risks of explosion of the product in presence of mechanical impact: No.
Fire Fighting Media
and Instructions
Solution 1, 65044A, Fixative SMALL FIRE: Use DRY chemical powder.
LARGE FIRE: Use alcohol foam, water spray or fog. Cool containing vessels with water jet in order to prevent pressure build-up, autoignition or explosion.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Protective Clothing (Fire)
Solution 1, 65044A, Fixative Be sure to use an approved/certified respirator or equivalent.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Special Remarks on Fire Hazards
Solution 1, 65044A, Fixative Dangerous fire and explosion risk. Container explosion may occur under fire conditions or when heated. Vapor may travel considerable distance to source of ignition and flash back.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Special Remarks on Explosion Hazards
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Forms explosion sensitive compounds with some metals such as lead, silver, mercury and copper. (Sodium Azide)
HARLECO® Hemacolor® Solution 3 Not available.

Section 6. Accidental Release Measures
Small Spill and Leak
Solution 1, 65044A, Fixative Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
Hemacolor Solution 2, Fixative Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
HARLECO® Hemacolor® Solution 3 Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
Large Spill and Leak
Solution 1, 65044A, Fixative Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside container. Do not touch spilled material. Use water spray to reduce vapors. Prevent entry into sewers, basements or confined areas; dike if needed. Call for assistance on disposal. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities.
Hemacolor Solution 2, Fixative Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities.
HARLECO® Hemacolor® Solution 3 Absorb with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and allow to evacuate through the sanitary system.
Spill Kit Information
Solution 1, 65044A, Fixative The following EMD Chemicals Inc. SpillSolv (TM) absorbent is recommended for this product:
SX1330 Solvent Treatment Kit
Hemacolor Solution 2, Fixative No specific spill kit required for this product.
HARLECO® Hemacolor® Solution 3 No specific spill kit required for this product.

Section 7. Handling and Storage
Handling
Solution 1, 65044A, Fixative Keep away from heat, sparks and flame. Keep container closed. Use only with adequate ventilation. To avoid fire or explosion, dissipate static electricity during transfer by grounding and bonding containers and equipment before transferring material. Use explosion-proof electrical (ventilating, lighting and material handling) equipment.
Hemacolor Solution 2, Fixative Avoid contact with eyes, skin and clothing. Avoid prolonged contact with eyes, skin, and clothing. Do not ingest. Avoid breathing vapors or spray mists. Keep container closed. Use only with adequate ventilation. Wash thoroughly after handling.
HARLECO® Hemacolor® Solution 3 Avoid contact with eyes, skin and clothing. Wash thoroughly after handling.
Storage
Solution 1, 65044A, Fixative Store in a segregated and approved area. Keep container in a cool, well-ventilated area. Keep container tightly closed and sealed until ready for use. Avoid all possible sources of ignition (spark or flame).
Hemacolor Solution 2, Fixative Keep container tightly closed. Keep container in a cool, well-ventilated area.
HARLECO® Hemacolor® Solution 3 Keep container tightly closed. Keep container in a cool, well-ventilated area.

Section 8. Exposure Controls/Personal Protection
Engineering Controls
Solution 1, 65044A, Fixative Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work-station location.
Hemacolor Solution 2, Fixative Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work-station location.
HARLECO® Hemacolor® Solution 3 Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits.
Personal Protection
Eyes
Solution 1, 65044A, Fixative Splash goggles.
Hemacolor Solution 2, Fixative Splash goggles.
HARLECO® Hemacolor® Solution 3 Splash goggles.
Body
Solution 1, 65044A, Fixative Lab coat.
Hemacolor Solution 2, Fixative Lab coat.
HARLECO® Hemacolor® Solution 3 Lab coat.
Respiratory
Solution 1, 65044A, Fixative Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Wear appropriate respirator when ventilation is inadequate.
Hemacolor Solution 2, Fixative Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Wear appropriate respirator when ventilation is inadequate.
HARLECO® Hemacolor® Solution 3 Not applicable.
Hands
Solution 1, 65044A, Fixative Gloves.
Hemacolor Solution 2, Fixative Gloves.
HARLECO® Hemacolor® Solution 3 Gloves.
Feet
Solution 1, 65044A, Fixative Not applicable.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Protective Clothing (Pictograms)
Splash goggles Lab coat Vapor Respirator Gloves
Personal Protection in Case of a Large Spill
Solution 1, 65044A, Fixative Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self-contained breathing apparatus should be used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
Hemacolor Solution 2, Fixative Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self-contained breathing apparatus should be used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
HARLECO® Hemacolor® Solution 3 Splash goggles. Full suit. Boots. Gloves. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
Product Name Exposure Limits
Methanol ACGIH (United States, 1994). Skin
TWA: 262 mg/m3
STEL: 328 mg/m3
OSHA (United States, 1989). Skin
TWA: 260 mg/m3
STEL: 325 mg/m3
ACGIH (United States, 1994). Skin
STEL: 328 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 262 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
NIOSH REL (United States, 1994). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 10 hour(s).
TWA: 200 ppm 10 hour(s).
OSHA Final Rule (United States, 1989). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
Water Not available.
Potassium Phosphate, Monobasic Not available.
Sodium Azide ACGIH (United States, 1996).
CEIL: 0.29 mg/m3
CEIL: 0.11 ppm Form: Vapor
NIOSH REL (United States, 1994). Skin
CEIL: 0.3 mg/m3 Form: As NaN3
CEIL: 0.1 ppm Form: As HN3
OSHA Final Rule (United States, 1989). Skin
CEIL: 0.3 mg/m3
CEIL: 0.1 ppm
Sodium Phosphate, Dibasic Not available.
Eosin Y Not available.
Methylene Blue Not available.
Azure B Not available.

Section 9. Physical and Chemical Properties
Odor
Solution 1, 65044A, Fixative Characteristic. Alcohol like.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Odorless.
Color
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Clear. Red.
HARLECO® Hemacolor® Solution 3 Purple.
Physical State and Appearance
Solution 1, 65044A, Fixative Liquid. (Colorless.)
Hemacolor Solution 2, Fixative Liquid.
HARLECO® Hemacolor® Solution 3 Liquid.
Molecular Weight
Solution 1, 65044A, Fixative Not applicable.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
Molecular Formula
Solution 1, 65044A, Fixative Not applicable.
Hemacolor Solution 2, Fixative Not applicable.
HARLECO® Hemacolor® Solution 3 Not applicable.
pH
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Boiling/Condensation Point
Solution 1, 65044A, Fixative 64.55°C (148.2°F)
Hemacolor Solution 2, Fixative The lowest known value is 99.9°C (211.8°F) (Water).
HARLECO® Hemacolor® Solution 3 The lowest known value is 99.9°C (211.8°F) (Water).
Melting/Freezing Point
Solution 1, 65044A, Fixative -97.72°C (-143.9°F)
Hemacolor Solution 2, Fixative May start to solidify at -0.1°C (31.8°F) based on data for: Water.
HARLECO® Hemacolor® Solution 3 May start to solidify at -0.1°C (31.8°F) based on data for: Water.
Critical Temperature
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Specific Gravity
Solution 1, 65044A, Fixative 0.792 (Water = 1)
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Vapor Pressure
Solution 1, 65044A, Fixative 12.9 kPa (97 mmHg) (@ 20°C)
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Vapor Density
Solution 1, 65044A, Fixative 1.11 (Air = 1)
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Volatility
Solution 1, 65044A, Fixative 99.9% (v/v).
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Odor Threshold
Solution 1, 65044A, Fixative 100 ppm
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Evaporation Rate
Solution 1, 65044A, Fixative 5.91 compared to (n-Butyl Acetate =1)
Hemacolor Solution 2, Fixative 0.36 (Water) compared to(n-Butyl Acetate =1)
HARLECO® Hemacolor® Solution 3 0.36 (Water) compared to(n-Butyl Acetate =1)
VOC
Solution 1, 65044A, Fixative 100 (%)
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Viscosity
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
LogKow
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Ionicity (in Water)
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Solubility
Solution 1, 65044A, Fixative Soluble in water.
Hemacolor Solution 2, Fixative Soluble in water.
HARLECO® Hemacolor® Solution 3 Easily soluble in water.
Physical Chemical Comments
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.

Section 10. Stability and Reactivity
Stability and Reactivity
Solution 1, 65044A, Fixative The product is stable.
Hemacolor Solution 2, Fixative The product is stable.
HARLECO® Hemacolor® Solution 3 The product is stable.
Conditions of Instability
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Incompatibility with Various Substances
Solution 1, 65044A, Fixative Highly reactive with oxidizing agents.
Reactive with metals, acids.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Rem/Incompatibility Contact with acids liberates toxic gas. (Sodium Azide)
Hazardous Decomposition Products
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Hazardous Polymerization
Solution 1, 65044A, Fixative Will not occur.
Hemacolor Solution 2, Fixative Will not occur.
HARLECO® Hemacolor® Solution 3 Will not occur.

Section 11. Toxicological Information
RTECS Number:
Solution 1, 65044A, Fixative PC1400000
Water ZC0110000
Potassium Phosphate TC6615500
Sodium Azide VY8050000
Sodium Phosphate, Dibasic, Anhydrous WC4500000
Eosin Y (Yellowish) LM5850000
Methylene Blue SO5600000
Azure B SO5550000
Toxicity
Solution 1, 65044A, Fixative Acute oral toxicity (LD50): 5628 mg/kg [Rat].
Acute dermal toxicity (LD50): 15800 mg/kg [Rabbit].
Acute toxicity of the vapor (LC50): 64000 ppm 4 hour(s) [Rat].
Hemacolor Solution 2, Fixative LD50: Not available.
LC50: Not available.
HARLECO® Hemacolor® Solution 3 LD50: Not available.
LC50: Not available.
Chronic Effects on Humans Not available.
Acute Effects on Humans
Solution 1, 65044A, Fixative Hazardous in case of eye contact (irritant). Inflammation of the eye is characterized by redness, watering, and itching. Hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Hazardous in case of inhalation (lung irritant). Extremely hazardous in case of ingestion. May be fatal if swallowed.
Hemacolor Solution 2, Fixative May be hazardous in case of eye contact (irritant). May be hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. May be hazardous in case of inhalation. May be hazardous in case of ingestion.
HARLECO® Hemacolor® Solution 3 May be hazardous in case of eye contact (irritant). May be hazardous in case of skin contact (irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
Special Remarks on Toxicity
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Special Remarks on Chronic Effects on Humans
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Special Remarks on Other Toxic Effects on Humans
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Synergetic Products (Toxicologically)
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Irritancy
Solution 1, 65044A, Fixative Draize Test ( Rabbit):
Skin: 20 mg/24h. Reaction: Moderate.
Draize Test (Rabbit) Eye: 100 mg/24h moderate
Hemacolor Solution 2, Fixative Draize Test: Not available.
HARLECO® Hemacolor® Solution 3 Draize Test: Not available.
Sensitization
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Carcinogenic Effects
Solution 1, 65044A, Fixative This material is not known to cause cancer in animals or humans.
Hemacolor Solution 2, Fixative This material is not known to cause cancer in animals or humans.
HARLECO® Hemacolor® Solution 3 This material is not known to cause cancer in animals or humans.
Toxicity to Reproductive System
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Teratogenic Effects
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Mutagenic Effects
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.

Section 12. Ecological Information
Ecotoxicity
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
BOD5 and COD
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Biodegradable/OECD
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Mobility
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Solution 1, 65044A, Fixative These products are carbon oxides (CO, CO2) and water.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Toxicity of the Products of Biodegradation
Solution 1, 65044A, Fixative The products of degradation are less toxic than the product itself.
Hemacolor Solution 2, Fixative The products of degradation are as toxic as the product itself.
HARLECO® Hemacolor® Solution 3 The products of degradation are more toxic than the product itself.
Special Remarks on the Products of Biodegradation
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.

Section 13. Disposal Considerations
EPA Waste Number
Solution 1, 65044A, Fixative U154 D001Specifed Technology - Contact your local permitted waste disposal site (TSD) for permissible treatment sites.Always contact permitted waste disposer (TSD) to asure compliance with all Current local, State and Federal Regulations.
Hemacolor Solution 2, Fixative Material does not have an EPA Waste number and is not a listed waste, however consultation with a permitted waste disposal site (TSD) should be accomplished.
HARLECO® Hemacolor® Solution 3 Material does not have an EPA Waste number and is not a listed waste, however consultation with a permitted waste disposal site (TSD) should be accomplished.
Treatment
Solution 1, 65044A, Fixative Incineration, fuels blending or recycle. Contact your local permitted waste disposal site (TSD) for permissible treatment sites. Always contact a permitted waste disposal (TSD) to assure compliance with all current local, state, and Federal Regulations.
Hemacolor Solution 2, Fixative Material does not have an EPA Waste number and is not a listed waste, however consultation with a permitted waste disposal site (TSD) should be accomplished.
HARLECO® Hemacolor® Solution 3 Material does not have an EPA Waste number and is not a listed waste, however consultation with a permitted waste disposal site (TSD) should be accomplished.

+ Section 14. Transport Information
DOT Classification Proper Shipping Name:Methanol Solution
Hazard Class: 3
UN number: UN1230
Packing Group: II
RQ:Not applicable.
TDG Classification Not available.
IMO/IMDG Classification Not available.
ICAO/IATA Classification Not available.

Section 15. Regulatory Information
U.S. Federal Regulations
Solution 1, 65044A, Fixative TSCA 8(b) inventory: Methanol
Hemacolor Solution 2, Fixative TSCA 8(b) inventory: Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y
HARLECO® Hemacolor® Solution 3 TSCA 8(b) inventory: Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue
Solution 1, 65044A, Fixative SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: Methanol
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Methanol: Fire Hazard, Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard
Hemacolor Solution 2, Fixative SARA 302/304/311/312 extremely hazardous substances: Sodium Azide
SARA 302/304 emergency planning and notification: Sodium Azide
SARA 302/304/311/312 hazardous chemicals: Sodium Azide; Sodium Phosphate, Dibasic
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Sodium Azide: Immediate (Acute) Health Hazard; Sodium Phosphate, Dibasic : Immediate (Acute) Health Hazard
HARLECO® Hemacolor® Solution 3 SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: Sodium Phosphate, Dibasic ; Methylene Blue
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Sodium Phosphate, Dibasic : Immediate (Acute) Health Hazard; Methylene Blue: Delayed (Chronic) Health Hazard
Solution 1, 65044A, Fixative SARA 313 toxic chemical notification and release reporting: Methanol
Hemacolor Solution 2, Fixative
HARLECO® Hemacolor® Solution 3 SARA 313 toxic chemical notification and release reporting: No products were found.
Solution 1, 65044A, Fixative Clean Water Act (CWA) 307: No products were found.
Hemacolor Solution 2, Fixative Clean Water Act (CWA) 307: No products were found.
HARLECO® Hemacolor® Solution 3 Clean Water Act (CWA) 307: No products were found.
Solution 1, 65044A, Fixative Clean Water Act (CWA) 311: No products were found.
Hemacolor Solution 2, Fixative Clean Water Act (CWA) 311: Sodium Phosphate, Dibasic
HARLECO® Hemacolor® Solution 3 Clean Water Act (CWA) 311: Sodium Phosphate, Dibasic
Solution 1, 65044A, Fixative Clean air act (CAA) 112 accidental release prevention: No products were found.
Hemacolor Solution 2, Fixative Clean air act (CAA) 112 accidental release prevention: No products were found.
HARLECO® Hemacolor® Solution 3 Clean air act (CAA) 112 accidental release prevention: No products were found.
Solution 1, 65044A, Fixative Clean air act (CAA) 112 regulated flammable substances: No products were found.
Hemacolor Solution 2, Fixative Clean air act (CAA) 112 regulated flammable substances: No products were found.
HARLECO® Hemacolor® Solution 3 Clean air act (CAA) 112 regulated flammable substances: No products were found.
Solution 1, 65044A, Fixative Clean air act (CAA) 112 regulated toxic substances: No products were found.
Hemacolor Solution 2, Fixative Clean air act (CAA) 112 regulated toxic substances: No products were found.
HARLECO® Hemacolor® Solution 3 Clean air act (CAA) 112 regulated toxic substances: No products were found.
WHMIS (Canada)
Solution 1, 65044A, Fixative CLASS B-2: Flammable liquid with a flash point lower than 37.8°C (100°F).
Class D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
Hemacolor Solution 2, Fixative Class D-2A: Material causing other toxic effects (VERY TOXIC).
HARLECO® Hemacolor® Solution 3 Not controlled under WHMIS (Canada).
Solution 1, 65044A, Fixative CEPA DSL: Methanol
Hemacolor Solution 2, Fixative CEPA DSL: Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y
HARLECO® Hemacolor® Solution 3 CEPA DSL: Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue
This product has been classifed in accordance with the hazard criteria of the Controlled Product Regulations and the MSDS contains all required information.
International Regulations
EINECS
Solution 1, 65044A, Fixative Methanol 200-659-6
Hemacolor Solution 2, Fixative Water 231-791-2
Potassium Phosphate, Monobasic 231-913-4
Sodium Azide 247-852-1
Sodium Phosphate, Dibasic 231-448-7
Eosin Y 241-409-6
HARLECO® Hemacolor® Solution 3 Water 231-791-2
Potassium Phosphate, Monobasic 231-913-4
Sodium Phosphate, Dibasic 231-448-7
Methylene Blue 200-515-2
Azure B 208-511-2
DSCL (EEC)
Solution 1, 65044A, Fixative R11- Highly flammable.
R37/38- Irritating to respiratory system and skin.
R41- Risk of serious damage to eyes.
Hemacolor Solution 2, Fixative R21- Harmful in contact with skin.
HARLECO® Hemacolor® Solution 3 This product is not classified according to the EU regulations.
International Lists
Solution 1, 65044A, Fixative Australia (NICNAS): Methanol

Japan (MITI): Methanol

Korea (TCCL): Methanol

Philippines (RA6969): Methanol
Hemacolor Solution 2, Fixative Australia (NICNAS): Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y

Japan (MITI): Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y

Korea (TCCL): Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y

Philippines (RA6969): Water; Potassium Phosphate, Monobasic; Sodium Azide; Sodium Phosphate, Dibasic ; Eosin Y
HARLECO® Hemacolor® Solution 3 Australia (NICNAS): Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue; Azure B

Japan (MITI): Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue

Korea (TCCL): Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue

Philippines (RA6969): Water; Potassium Phosphate, Monobasic; Sodium Phosphate, Dibasic ; Methylene Blue
Solution 1, 65044A, Fixative China: No products were found.
Hemacolor Solution 2, Fixative China: No products were found.
HARLECO® Hemacolor® Solution 3 China: No products were found.
State Regulations
Solution 1, 65044A, Fixative Pennsylvania RTK: Methanol: (environmental hazard, generic environmental hazard)
Massachusetts RTK: Methanol
New Jersey: Methanol
Hemacolor Solution 2, Fixative Pennsylvania RTK: Sodium Azide: (environmental hazard, generic environmental hazard); Sodium Phosphate, Dibasic : (environmental hazard, generic environmental hazard)
Massachusetts RTK: Sodium Azide; Sodium Phosphate, Dibasic
New Jersey: Hemacolor Solution 2, Fixative
HARLECO® Hemacolor® Solution 3 Pennsylvania RTK: Sodium Phosphate, Dibasic : (environmental hazard, generic environmental hazard)
Massachusetts RTK: Sodium Phosphate, Dibasic
New Jersey: HARLECO® Hemacolor® Solution 3
Solution 1, 65044A, Fixative California prop. 65: No products were found.
Hemacolor Solution 2, Fixative California prop. 65: No products were found.
HARLECO® Hemacolor® Solution 3 California prop. 65: No products were found.

Section 16. Other Information
National Fire Protection Association (U.S.A.)
Health
1 3 0
Fire Hazard

Reactivity
Specific Hazard
Other Special Considerations
Solution 1, 65044A, Fixative Not available.
Hemacolor Solution 2, Fixative Not available.
HARLECO® Hemacolor® Solution 3 Not available.
Changed Since Last Revision +
Notice to Reader
The statements contained herein are based upon technical data that EMD Chemicals Inc. believes to be reliable, are offered for information purposes only and as a guide to the appropriate precautionary and emergency handling of the material by a properly trained person having the necessary technical skills. Users should consider these data only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use, storage and disposal of these materials and the safety and health of employees and customers and the protection of the environment. EMD CHEMICALS INC. MAKES NO REPRESENTATION OR WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE, WITH RESPECT TO THE INFORMATION HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS.