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Material Safety Data Sheet
Section 1. Product and Company Identification
Product Name Wright Giemsa Stain Solution, HARLECO ® Product Code 742
Manufacturer
EMD Chemicals Inc.
P.O. Box 70
480 Democrat Road
Gibbstown, NJ 08027
Prior to January 1, 2003 EMD Chemicals Inc. was EM Industries, Inc. or EM Science, Division of EM Industries, Inc.
Effective Date
3/11/2005
For More Information Call In Case of Emergency Call
856-423-6300 Technical Service
Monday-Friday: 8:00 AM - 5:00 PM
800-424-9300 CHEMTREC (USA)
613-996-6666 CANUTEC (Canada)
24 Hours/Day: 7 Days/Week
Synonym None.
Material Uses Analytical reagent.
Chemical Family Solution.


Section 2. Composition and Information on Ingredients
Component CAS # % by Weight
Wright Stain 68988-92-1 0-1
Glycerin 56-81-5 0-20
Methanol 67-56-1 80-99
Giemsa's Stain 51811-82-6 0-1


Section 3. Hazards Identification
Physical State and Appearance Liquid.
Emergency Overview DANGER !
POISON !
FLAMMABLE LIQUID AND VAPOR.
VAPOR MAY CAUSE FLASH FIRE.
MAY BE FATAL IF SWALLOWED.
MAY CAUSE BLINDNESS IF SWALLOWED.
CANNOT BE MADE NON-POISONOUS.
HARMFUL IF INHALED OR ABSORBED THROUGH SKIN.
CAUSES RESPIRATORY TRACT, EYE AND SKIN IRRITATION.
CONTAINS MATERIAL WHICH CAUSES DAMAGE TO THE FOLLOWING ORGANS: KIDNEYS, GASTROINTESTINAL TRACT, RESPIRATORY TRACT, SKIN, CENTRAL NERVOUS SYSTEM, EYE, LENS OR CORNEA.
Routes of Entry Absorbed through skin. Dermal contact. Eye contact. Inhalation. Ingestion.
Potential Acute Health Effects
Eyes Hazardous in case of eye contact (irritant). Inflammation of the eye is characterized by redness, watering, and itching.
Skin Hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering.
Inhalation Hazardous in case of inhalation (lung irritant).
Ingestion Extremely hazardous in case of ingestion. MAY BE FATAL OF CAUSE BLINDNESS IF SWALLOWED.
Potential Chronic Health Effects
Carcinogenic Effects This material is not known to cause cancer in animals or humans.
Additional information See Toxicological Information (section 11)
Medical Conditions Aggravated by Overexposure: Repeated exposure to a highly toxic material may produce general deterioration of health by an accumulation in one or many human organs.


Section 4. First Aid Measures
Eye Contact Check for and remove any contact lenses. In case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Cold water may be used. Get medical attention immediately.
Skin Contact In case of contact, immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Cover the irritated skin with an emollient. Cold water may be used. Wash clothing before reuse. Thoroughly clean shoes before reuse. Get medical attention immediately.
Inhalation If inhaled, remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately.
Ingestion If swallowed, do not induce vomiting unless directed to do so by medical personnel. Never give anything by mouth to an unconscious person. Loosen tight clothing such as a collar, tie, belt or waistband. Get medical attention immediately.


Section 5. Fire Fighting Measures
Flammability of the Product Product will burn.
Auto-ignition Temperature The lowest known value is 369.9°C (697.8°F) (Glycerin).
Flash Points Closed cup: 12.222°C (54°F).
Flammable Limits The greatest known range is LOWER: 6% UPPER: 36.5% (Methanol)
Products of Combustion These products are carbon oxides (CO, CO2).
Fire Hazards in Presence of Various Substances Highly flammable in presence of open flames, sparks and static discharge, of shocks, of heat, of oxidizing materials.
Explosion Hazards in Presence of Various Substances Risks of explosion of the product in presence of static discharge:
Highly flammable in presence of open flames, sparks and static discharge.
Highly explosive in presence of open flames, sparks and static discharge.

Risks of explosion of the product in presence of mechanical impact:
Highly flammable in presence of shocks.
Highly explosive in presence of shocks.
Fire Fighting Media
and Instructions
SMALL FIRE: Use DRY chemical powder.
LARGE FIRE: Use alcohol foam, water spray or fog. Cool containing vessels with water jet in order to prevent pressure build-up, autoignition or explosion.
Protective Clothing (Fire) Be sure to use an approved/certified respirator or equivalent.
Special Remarks on Fire Hazards Dangerous fire and explosion risk. Container explosion may occur under fire conditions or when heated. Vapor may travel considerable distance to source of ignition and flash back. (Methanol)
Special Remarks on Explosion Hazards Not available.


Section 6. Accidental Release Measures
Small Spill and Leak Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container.
Large Spill and Leak Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not get water inside container. Do not touch spilled material. Use water spray to reduce vapors. Prevent entry into sewers, basements or confined areas; dike if needed. Call for assistance on disposal. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities.
Spill Kit Information The following EMD Chemicals Inc. SpillSolv (TM) absorbent is recommended for this product: SX1330 Solvent Treatment Kit


Section 7. Handling and Storage
Handling Keep away from heat, sparks and flame. Keep container closed. Avoid breathing vapors or spray mists. Do not get in eyes, on skin, or on clothing. Do not ingest.
Storage Keep container in a cool, well-ventilated area.


Section 8. Exposure Controls/Personal Protection
Engineering Controls Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective occupational exposure limits. Ensure that eyewash stations and safety showers are proximal to the work-station location.
Personal Protection
Eyes Splash goggles.
Body Lab coat.
Respiratory Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Wear appropriate respirator when ventilation is inadequate.
Hands Gloves.
Feet Not applicable.
Protective Clothing (Pictograms)
Safety glasses Lab coat Vapor Respirator Gloves
Personal Protection in Case of a Large Spill Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self-contained breathing apparatus should be used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product.
Product Name Exposure Limits
Wright Stain Not available.
Glycerin Belgium Minister of Labour (Belgium, 1998).
VL: 10 mg/m3 8 hour(s).
Tyterveyslaitos (Finland, 1998).
TWA: 20 mg/m3 8 hour(s).
INRS (France, 1996).
VME: 10 mg/m3 8 hour(s).
National Authority for Occupational Safety/Health (Ireland, 1999).
OEL: 10 mg/m3 8 hour(s).
Arbeidsinspectie (Netherlands, 1999).
TGG 8 uur: 10 mg/m3 8 hour(s).
EH40-OES (United Kingdom (UK), 1997).
TWA: 10 mg/m3 8 hour(s).
ACGIH (United States, 1994).
TWA: 10 mg/m3 8 hour(s). Form: Mist
OSHA Final Rule (United States, 1989).
TWA: 5 mg/m3 8 hour(s). Form: Respirable fraction
TWA: 10 mg/m3 8 hour(s). Form: Total dust
Methanol ACGIH (United States, 1994). Skin
TWA: 262 mg/m3
STEL: 328 mg/m3
OSHA (United States, 1989). Skin
TWA: 260 mg/m3
STEL: 325 mg/m3
ACGIH (United States, 1994). Skin
STEL: 328 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 262 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
NIOSH REL (United States, 1994). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 10 hour(s).
TWA: 200 ppm 10 hour(s).
OSHA Final Rule (United States, 1989). Skin
STEL: 325 mg/m3 15 minute(s).
STEL: 250 ppm 15 minute(s).
TWA: 260 mg/m3 8 hour(s).
TWA: 200 ppm 8 hour(s).
Giemsa's Stain Not available.


Section 9. Physical and Chemical Properties
Odor Slight Alcohol like.
Color Purple.
Physical State and Appearance Liquid.
Molecular Weight Not applicable.
Molecular Formula Not applicable.
pH Not available.
Boiling/Condensation Point The lowest known value is 64.55°C (148.2°F) (Methanol). Weighted average: 87.21°C (189°F)
Melting/Freezing Point May start to solidify at 17.8°C (64°F) based on data for: Glycerin. Weighted average: -86.11°C (-123°F)
Specific Gravity Weighted average: 0.82 (Water = 1)
Vapor Pressure The highest known value is 12.9 kPa (97 mmHg) (@ 20°C) (Methanol).
Vapor Density The highest known value is 3.1 (Air = 1) (Glycerin). Weighted average: 1.31 (Air = 1)
Volatility 99.9% (v/v). (Methanol.)
Odor Threshold The lowest known value is 100 ppm (Methanol)
Evaporation Rate 5.91 (Methanol) compared to (n-BUTYL ACETATE=1)
VOC 100 (%)
LogKow Not available.
Solubility Soluble in water.


Section 10. Stability and Reactivity
Stability and Reactivity The product is stable.
Conditions of Instability Not available.
Incompatibility with Various Substances Highly reactive with oxidizing agents.
Reactive with metals, acids.
Rem/Incompatibility Not available.
Hazardous Decomposition Products carbon oxides (CO, CO2)
Hazardous Polymerization Will not occur.


Section 11. Toxicological Information
RTECS Number:
Wright Stain Not available.
Glycerol MA8050000
Methanol PC1400000
Giemsa Stain Not available.
Toxicity Acute oral toxicity (LD50): 4090 mg/kg [Mouse]. (Glycerin).
Acute dermal toxicity (LD50): 15800 mg/kg [Rabbit]. (Methanol).
Acute toxicity of the vapor (LC50): 64000 ppm 4 hour(s) [Rat]. (Methanol).
Chronic Effects on Humans Not available.
Acute Effects on Humans Hazardous in case of eye contact (irritant). Inflammation of the eye is characterized by redness, watering, and itching. Hazardous in case of skin contact (permeator, irritant). Skin inflammation is characterized by itching, scaling, reddening, or, occasionally, blistering. Hazardous in case of inhalation (lung irritant). Extremely hazardous in case of ingestion. May be fatal if swallowed.
Synergetic Products (Toxicologically) Not available.
Irritancy Draize Test: Not available.
Sensitization Not available.
Carcinogenic Effects This material is not known to cause cancer in animals or humans.
Toxicity to Reproductive System Not available.
Teratogenic Effects Not available.
Mutagenic Effects Not available.


Section 12. Ecological Information
Ecotoxicity Not available.
BOD5 and COD Not available.
Toxicity of the Products of Biodegradation The products of degradation are less toxic than the product itself.


Section 13. Disposal Considerations
EPA Waste Number D001 U154
Treatment Incineration, fuels blending or recycle. Contact your local permitted waste disposal site (TSD) for permissible treatment sites. Always contact a permitted waste disposal (TSD) to assure compliance with all current local, state, and Federal Regulations..


Section 14. Transport Information
DOT Classification Proper Shipping Name: METHANOL SOLUTION
Hazard Class: 3
UN number: UN1230
Packing Group: II
RQ: Not applicable.
DOT Class 3 - Flammable liquid
TDG Classification Not available.
IMO/IMDG Classification Proper Shipping Name: METHANOL SOLUTION
Hazard Class: 3
UN number: UN1230
Packing Group: II
RQ: Not applicable.
ICAO/IATA Classification Not available.


Section 15. Regulatory Information
U.S. Federal Regulations TSCA 8(b) inventory: Wright Stain; Glycerin; Methanol; Giemsa's Stain
SARA 302/304/311/312 extremely hazardous substances: No products were found.
SARA 302/304 emergency planning and notification: No products were found.
SARA 302/304/311/312 hazardous chemicals: Glycerin; Methanol
SARA 311/312 MSDS distribution - chemical inventory - hazard identification: Glycerin: Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard; Methanol: Fire Hazard, Immediate (Acute) Health Hazard, Delayed (Chronic) Health Hazard
SARA 313 toxic chemical notification and release reporting: Methanol 89.5%
Clean Water Act (CWA) 307: No products were found.
Clean Water Act (CWA) 311: No products were found.
Clean air act (CAA) 112 accidental release prevention: No products were found.
Clean air act (CAA) 112 regulated flammable substances: No products were found.
Clean air act (CAA) 112 regulated toxic substances: No products were found.
WHMIS (Canada) CLASS B-2: Flammable liquid with a flash point lower than 37.8°C (100°F).
Class D-1A: Material causing immediate and serious toxic effects (VERY TOXIC).
Class D-2B: Material causing other toxic effects (TOXIC).
CEPA DSL: Wright Stain; Glycerin; Methanol; Giemsa's Stain
This product has been classifed in accordance with the hazard criteria of the Controlled Product Regulations and the MSDS contains all required information.
International Regulations
EINECS Wright Stain 273-541-5
Glycerin 200-289-5
Methanol 200-659-6
Giemsa's Stain 257-438-2
DSCL (EEC) R11- Highly flammable.
R37/38- Irritating to respiratory system and skin.
R41- Risk of serious damage to eyes.
International Lists Australia (NICNAS): Wright Stain; Glycerin; Methanol; Giemsa's Stain

Japan (MITI): Glycerin; Methanol

Korea (TCCL): Glycerin; Methanol

Philippines (RA6969): Wright Stain; Glycerin; Methanol; Giemsa's Stain
China: No products were found.
State Regulations Pennsylvania RTK: Glycerin: (generic environmental hazard); Methanol: (environmental hazard, generic environmental hazard)
Massachusetts RTK: Glycerin; Methanol
New Jersey: Methanol
California prop. 65: No products were found.


Section 16. Other Information
National Fire Protection Association (U.S.A.)
Health
1 3 0
Fire Hazard

Reactivity

Specific Hazard
Changed Since Last Revision +
Notice to Reader
The statements contained herein are based upon technical data that EMD Chemicals Inc. believes to be reliable, are offered for information purposes only and as a guide to the appropriate precautionary and emergency handling of the material by a properly trained person having the necessary technical skills. Users should consider these data only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use, storage and disposal of these materials and the safety and health of employees and customers and the protection of the environment. EMD CHEMICALS INC. MAKES NO REPRESENTATION OR WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE, WITH RESPECT TO THE INFORMATION HEREIN OR THE PRODUCT TO WHICH THE INFORMATION REFERS.




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